QuantiFluor® ssDNA System

Simple, Accurate DNA Quantitation

The QuantiFluor® ssDNA System contains a fluorescent dye that enables sensitive quantitation of small amounts of single-stranded (ssDNA) in solution. Detecting and quantitating ssDNA is useful many molecular biology research applications, including studying ssDNA viruses, quantitating short synthetic ssDNA probes for site-directed mutagenesis, analysis of first-strand cDNAs, and quantitating bisulfate-treated DNA to study DNA methylation.

The QuantiFluor® ssDNA System requires less DNA (200pg) than other methods (~1 µg), so you save more of your sample for downstream assays.

Improved Sensitivity vs. Absorbance and Other Fluorescent ssDNA Quantitation Systems

For low-concentration samples, sensitivity of the QuantiFluor® ssDNA System is significantly increased compared to absorbance at 260nm (NanoDrop® method) and Qubit® ssDNA.

The dynamic range of the assay is wider than that of Qubit® ssDNA and equivalent to OliGreen® reagent.

	Sample*	Assay
QuantiFluor® ssDNA System	0.2–400ng/µl	0.2–400ng
Quant-iT™ OliGreen	0.2–400ng/µl	0.2–400ng
Qubit® ssDNA	1–200ng/µl	1–200ng
NanoDrop® 2000 Spectrophotometer	2–12,000ng/µl	2–12,000ng

*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input dsDNA per assay.

ssDNA Quantitation Sensitivity and Assay Linearity

The QuantiFluor® ssDNA System detects as little as 1ng/ml ssDNA in a 96-well microplate assay (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU. Sensitivity is equivalent to Quanti-IT™ OliGreen® reagent.

Easy Setup on Microplate or Single-Tube Fluorometers

The QuantiFluor® ssDNA System includes all the necessary reagents to quickly set up and quantitate single-stranded DNA, and is easy to set up on microplate or single-tube fluorometers. The System is compatible with any fluorometer capable of measuring the appropriate fluorescence excitation and emission spectra. Compatible Promega fluorometers include:

Quantus Nucleic Acid Quantification Fluorometer

Quantus™ Fluorometer

(Use Blue Channel Ex 460nm, Em 515–575nm)

Discover Nanodrop Spectrophotometer DNA Extraction

GloMax® Discover

Explorer DNA Purification & Quantification Kit

GloMax® Explorer

How Do Fluorescent Dyes Compare to Other Quantitation Methods?

In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.

UV-Absorbance vs Fluorescence

Fluorescence vs qPCR

Protocols

Complete Protocol

QuantiFluor® ssDNA System Technical ManualPDF (1306 KB) – English

Quick Protocols

QuantiFluor ssDNA System Quick Protocol FB198PDF (750 KB) – English

What's in the box?

Item	Part #	Size	Available Separately
20X TE Buffer (pH 7.5)	E260A	1 × 25ml	View Product
QuantiFluor® ssDNA Dye, 200X	E284A	1 × 1ml
ssDNA Standard	E288A	1 × 100μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

QuantiFluor® ssDNA System

Sensitive Quantitation of Single-Stranded DNA

Simple, Accurate DNA Quantitation

Improved Sensitivity vs. Absorbance and Other Fluorescent ssDNA Quantitation Systems

ssDNA Quantitation Sensitivity and Assay Linearity

Easy Setup on Microplate or Single-Tube Fluorometers