ProNex® Size-Selective Purification System
Superior Size Selection and Recovery for NGS and PCR
- High specificity of size selection with low carryover of unwanted DNA
- Exceptional DNA recovery
- Fast magnetic response time and low viscosity for accurate pipetting
Catalog Number:
Size
$ 344.00
Catalog Number: NG2001
$ 1,435.00
Catalog Number: NG2002
$ 3,442.00
Catalog Number: NG2003
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Efficient, Accurate Size Selection
The ProNex® Size-Selective Purification System enables the rapid and efficient magnetic resin-based purification of double-stranded DNA (dsDNA) for next-generation sequencing (NGS), polymerase chain reaction (PCR) and general molecular biology applications. The ProNex® System allows users to select the desired size of purified dsDNA fragments, from 100bp to 750bp. The novel reagent formulation provides significantly improved selectivity, reproducibility and yield relative to traditional dsDNA purification methods. In addition, the ProNex® System can be used in both manual and automated high-throughput workflows.
Control Your DNA Size
By adding varying ratios of ProNex® Size Selection Chemistry to your sample, you can control what size DNA is retained or eliminated. This enables NGS library and PCR fragment cleanup, as well as dual size selection of libraries.
Superior DNA Recovery
Serial buffer exchange/cleanup purification comparison between ProNex® Size-Selective Purification System and AMPure® XP beads. Five sequential cleanup rounds (AMPure® XP = 1.8X ratio, ProNex® = 3X ratio) were performed on a 200bp DNA step ladder. Ratios designed to retain all fragments. N=8 replicates per sample.
Easy to Pipette with Fast Bead Response
Significantly Lower Viscosity—No Sticking to Tips
5X Greater Bead Response Time—See the Difference
More Accurate Dual Library Size Selection
Getting the most sequencing data for your money requires precise size selection of your library. Elimination of both small and large fragments outside the desired range is critical to avoid wasting money on unusable reads. The ProNex® Size Selective Purification System not only removes more low- and high-molecular weight DNA than existing methods but also retains more of the target fragment sizes you really want.
E. coli libraries were created using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, using starting input values of 1,000ng, 100ng, 10ng and 1ng. Libraries were size selected with either AMPure® XP beads or the ProNex® Size-Selective Purification System. Libraries were centered at 300bp, using recommended library kit manufacturer’s recommendations for size selection with AMPure® XP beads and Promega’s guidelines for the ProNex® Size-Selective Purification System. Post-PCR libraries are shown. Libraries generated using the ProNex® System significantly reduced high-molecular weight DNA carryover and produced tighter size distribution.
Significantly Higher Library Yields
Total library yield comparison between ProNex® Size-Selective Purification System and AMPure® XP beads. E. coli libraries were created using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, using starting input values of 1,000ng, 100ng, 10ng and 1ng. Libraries were size selected with either AMPure® XP beads or the ProNex® Size-Selective Purification System. Libraries were centered at 300bp, using recommended library kit manufacturer’s recommendations for size selection with AMPure® XP beads and Promega’s guidelines for the ProNex® Size-Selective Purification System. Total yield of post size-selection libraries are shown.
Better Library Prep = The Sequencing Data You Want
ProNex® Chemistry delivers tighter distribution of desired fragment sizes with minimal fragments outside of your chosen range. A more narrow distribution means fewer wasted reads and more efficient sequencing.
Comparison of Library Fragment Size Distributions
Comparison of Sequencing Metrics (1000ng Sample Inputs)
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
ProNex® Size-Selective Chemistry |
NG103A | 1 × 10ml | |
Wash Buffer |
NG105A | 1 × 15ml | View Product |
Elution Buffer (10mM Tris [pH 8.5]) |
NG116A | 1 × 10ml |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
ProNex® Size-Selective Chemistry |
NG103B | 1 × 125ml | |
Wash Buffer |
NG105B | 1 × 180ml | View Product |
Elution Buffer (10mM Tris [pH 8.5]) |
NG116B | 1 × 125ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
ProNex® Size-Selective Chemistry |
NG103B | 4 × 125ml | |
Wash Buffer |
NG1051 | 2 × 340ml | View Product |
Elution Buffer (10mM Tris [pH 8.5]) |
NG116B | 4 × 125ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
Resources
Featured Resource
Poster: ProNex® Size-Selective Purification System for dsDNA fragment purification and automation on the CyBio FeliX from Analytik Jena
Articles
Other Resources
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