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Methylation Sensitivity of Isoschizomer Neoschizomer Pairs
For ordering information on the products discussed here, please visit our Restriction Enzymes product listing.
Isoschizomers and neoschizomers often differ in their sensitivity to methylation. This can be a simple way to differentiate the methylation state of DNA. Iso/neoschizomer pairs sold by Promega are listed below.
| Methylated Sequence | Cleaved by | Not Cleaved by |
|---|---|---|
| m4CCGG | MspI (C/CGG) | HpaII (C/CGG) |
| Cm5CGG | MspI (C/CGG) | HpaII (C/CGG) |
| Cm4CGG | MspI (C/CGG) | HpaII (C/CGG) |
| CCm5CGGG | XmaI (C/CCGGG) | SmaI (CCC/GGG) |
| Gm6ATC | Sau3AI (/GATC) | MboI, NdeII (/GATC) |
| GATm5C | MboI, NdeII (/GATC) | Sau3AI (/GATC) |
| GATm4C | MboI (/GATC) | Sau3AI (/GATC) |
| GGTACm5C | KpnI (GGTAC/C) | Acc65I (G/GTACC) |
Reference
McClelland, M., Nelson, M. and Raschke, E. (1994) Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nucl. Acids Res. 22, 3640.