Alkaline Phosphatase, Calf Intestinal (CIAP) Protocol
Instructions for Use of Product(s)
M1821, M2825
Literature # 9PIM182
Calf intestinal alkaline phosphatase (CIAP) catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vehicle DNA by removing phosphate groups from both 5´-termini.
Revised 4/18.