GoTaq® DNA Polymerase (M300) Protocol
Instructions for Use of Product(s)
M3001, M3005, M3008
Literature # 9PIM300
GoTaq® DNA Polymerase contains GoTaq® DNA Polymerase, 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer. The enzyme is a Taq DNA polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. The 5X Green GoTaq® Reaction Buffer contains two dyes (blue and yellow) that separate during electrophoresis to monitor migration progress. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR. Both buffers contain MgCl2 at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction.
Summary of Changes
The following changes were made to the 10/23 revision of this document:
1. Expired patent statements were removed.
2. Protocol template and font were updated.
Revised 10/23.