Griess Reagent System Technical Bulletin
Instructions for Use of Product(s)
G2930
Literature # TB229
The Griess Reagent System is based on the chemical diazotization reaction that was originally described by Griess in 1879, which uses sulfanilamide and N-1-napthylethylenediamine dihydrochloride (NED) under acidic (phosphoric acid) conditions. This system detects NO2– in a variety of biological and experimental liquid matrices such as plasma, serum, urine and tissue culture medium. The nitrite sensitivity depends on the matrix. The limit of detection is 2.5μM (125pmol) nitrite (in ultrapure, deionized distilled water).
Summary of Changes
The following changes were made to the 10/24 revision of this document:
1. Contents moved to latest TB template, cover image and fonts were updated.
2. Removed citations list in Section 1. Find citations by searching at: www.promega.com/citations/
3. Updated Section 2 heading and storage temperatures to reflect current styles.
4. Updated Section 7, Related Products.
Revised 10/24.