Raji (LDH-HiBiT) TCK Bioassays Technical Manual
Instructions for Use of Product(s)
JA1221, JA1225, JA1231, JA1235, JA1241, JA1245, JA1311, JA1315
Literature # TM748
Raji (LDH-HiBiT) TCK Bioassays measure the death of Raji cells engineered to express a fusion of HiBiT to the C terminus of the lactate dehydrogenase (LDHB) gene product (LDH-HiBiT). HiBiT is an 11 amino acid peptide that binds with high affinity to LgBiT to form NanoBiT® luciferase. When dead or dying cells release the HiBiT fusion protein into the extracellular medium, it binds to the cell-impermeable LgBiT, which is provided with the furimazine substrate in a nonlytic, homogeneous detection reagent. In coculture experiments, the luminescent signal will be proportional to target cell death alone, with no signal contribution arising from the death of effector cells.
Raji (LDH-HiBiT) TCK Bioassays are a suite of four individual bioassays comprised of the parental Raji line and three different knockout (KO) variants. Each bioassay contains a clonal cell line in the thaw-and-use format, which can be thawed, plated and used in assays without the need for continuous cell culture. All cell lines express the LDH-HiBiT reporter, making them suitable for analysis using the HiBiT TCK technology.
This Technical Manual is relevant for Cat.# JA1311, JA1315, JA1221, JA1225, JA1231, JA1235, JA1241 and JA1245; JA1611, JA1615, JA1621, JA1625, JA1631 and JA1635 (Korea).
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