Developmental Validation of a Real-Time PCR Assay for the Simultaneous Quantification of Total Human and Male DNA

Abstract

Multiplex human short tandem repeat analysis demands reliable DNA quantification to consistently produce interpretable genotypes. The Plexor® HY System is a multiplex quantitative PCR assay to quantify total human and male DNA. We performed developmental validation of the Plexor® HY System to demonstrate the performance capabilities and limitations of the assay for forensic applications. Validation studies examined: a) human specificity, b) sensitivity, c) quantification of degraded DNA, d) impact of inhibitors, e) male/female mixture and Y-assay male specificity, f) reproducibility and concordance and g) population studies.