Measuring JumonjiC Demethylase and Fe(II)/alpha-KG-Dependent Hydroxylase Activities Using the Succinate-Glo™ Assay and GloMax® Discover System
Publication Date: June 2017
Abstract
The Succinate-Glo™ Assay is a universal assay for detection of Fe(II)/α-KG-dependent hydroxylase and JumonjiC domain-containing histone lysine demethylase activities. The assay relies on enzymatic conversion of the succinate produced by the Fe(II)/α-KG-dependent hydroxylase reaction into ATP followed by bioluminescent detection of the created ATP. Measuring the luminescence from the Succinate-Glo™ Assay is easy on the GloMax® Discover System because the protocol comes preloaded on the instrument.
Related Products
How to Cite This Article
Scientific Style and Format, 7th edition, 2006
Promega Corporation Measuring JumonjiC Demethylase and Fe(II)/alpha-KG-Dependent Hydroxylase Activities Using the Succinate-Glo™ Assay and GloMax® Discover System. [Internet] June 2017. [cited: year, month, date]. Available from: https://www.promega.com/resources/pubhub/applications-notes/measure-jmjcdemethylasefeii-alpha-kg-hydroxylase-activitysuccinate-glo-assay-and-glomax-discover/
American Medical Association, Manual of Style, 10th edition, 2007
Promega Corporation Measuring JumonjiC Demethylase and Fe(II)/alpha-KG-Dependent Hydroxylase Activities Using the Succinate-Glo™ Assay and GloMax® Discover System. Promega Corporation Web site. https://www.promega.com/resources/pubhub/applications-notes/measure-jmjcdemethylasefeii-alpha-kg-hydroxylase-activitysuccinate-glo-assay-and-glomax-discover/ Updated June 2017. Accessed Month Day, Year.
