Six Luminescent Substrates for Cytochrome P450 Assays
Promega Corporation
Publication Date: 2009
Publication Date: 2009
Abstract
Luminogenic CYP assays use prosubstrates for the light-generating reaction of firefly luciferase. CYPs convert the prosubstrates to an active luciferin, which produces light in a second reaction with luciferase. The amount of light generated is proportional to the amount of luciferin produced by the CYP and, therefore, to CYP enzyme activity.
Cell Notes 23, 19–20.
