A Bioluminescent Assay for Calpain Activity
Martha O’Brien1, Mike Scurria2, Kay Rashka1, Bill Daily2 and Terry Riss1
1Promega Corporation and 2Promega Biosciences, Inc.
Publication Date: 2005
1Promega Corporation and 2Promega Biosciences, Inc.
Publication Date: 2005
Abstract
We have developed an exceptionally sensitive assay for the proteases calpain I and II. The Calpain-Glo™ Protease Assay is a rapid, homogeneous assay with a luminescent signal that is proportional to the amount of calpain present in the sample. The Calpain-Glo™ Assay uses a recombinant thermostable luciferase, Ultra-Glo™ Luciferase, that dramatically improves sensitivity and detects calpain over a broad range of activity.
Promega Notes 91, 6–9.
Learn about the chemistry of the bioluminescent reaction and its use in a broad range of assays.
