ProTEV Protease for Fusion Protein Processing

Jessica Anderson, Jami English and John Shultz
Promega Corporation
Publication Date: 2007

Abstract

ProTEV Protease is an improved 50 kDa version of the NIa protease from tobacco etch virus (TEV) that has been engineered to be more stable than native TEV protease for prolonged enzyme activity. It is used primarily to cleave affinity tags from fusion proteins during or after affinity purification. ProTEV Protease is active over a broad pH and temperature range to allow tailoring of cleavage conditions for the fusion protein of interest.

Promega Notes 97, 22–23.