HaloTag® Technology: A Powerful Tool for Protein Labeling and Analysis
Start by adding the HaloTag® protein fusion tag to your protein, pair the fusion protein with one of many interchangeable ligands that forms a highly specific covalent bond with HaloTag, and you open a gateway to investigate protein function. Once your protein of interest is fused to the HaloTag® protein, you are ready for numerous applications including protein purification, protein:protein or protein:DNA interactions, cellular imaging, receptor internalization and more. Each possible analysis pathway is based on which interchangeable HaloTag® ligand you select, or customize a ligand for your specific requirements.
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Find Your HaloTag® Protein Analysis Tools
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Protein Purification from E. coli and Mammalian Cells
Learn how to purify your protein fused to HaloTag from live cells and cell lysates.
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Protein Localization, Imaging, Trafficking and Turnover
Track where proteins are in the cell, where they are going and how long they stay using fixed and live cells.
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Protein:Protein and Protein:DNA Interaction Analysis
Study how proteins and protein complexes interact in live cells or examine DNA:protein interactions without antibodies.
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Super Resolution Microscopy
Follow a single molecule in cells when using a bright Janelia Fluor® ligand with a HaloTag® fusion protein.
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HaloTag® Clones
Thousands of clones that have been validated by:
- Fluorescent imaging
- DNA sequencing
- Protein expression