Notes: These authors investigated silicon dioxide nanoparticles (SiNPs) as a potential cancer treatment and identified the mechanism mediating cancer cell death. The Caspase-Glo® 3/7, Caspase-Glo® 9, CellTiter-Glo® luminescent cell-viability, ROS-Glo™, and Caspase-Glo® 1 assays were used to monitor cellular response to SiNPs. Together, SiNPs were identified to activate the intrinsic apoptosis pathway and lead to cell death.

  (5165)

Notes: RNA was extracted from the cells collected from differentiating hESC and hiPSC at days 0, 9, and 20 using the ReliaPrep RNA Cell Miniprep System. The RNA quality was evaluated using the Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, MA). One microgram of extracted RNA was converted into cDNA using reverse transcription (GoScript Transcription System). Quantitative real‐time polymerase chain reaction (qRT‐PCR) was carried out using the QuantStudio 7 Flex Real‐Time PCR System (Thermo Fisher Scientific, MA) and GoTaq qPCR Master Mix. pGL3 BRE Luciferase was used for plasmid lipofection to transfect the cells in each well of a 24-well plate. Cells that were transfected with empty vector (pGL3‐Basic) or BMP reporter (pGL3 BRE Luciferase) were cotransfected with empty Renilla vector (pRL‐Null). Luciferase activities were evaluated with a Dual‐Luciferase Assay System. (4982)

Notes: ReliaPrep RNA Cell Miniprep System was used to isolate Total RNA from peritoneal exudate cells of probiotics. Then the RNA was reverse transcribed to generate first-strand cDNA and RT-qPCR was performed using non-promega products. (4981)

Notes: Total RNA was isolated from normal human diploid fibroblast TIG-3 cells with a ReliaPrep RNA Cell MiniPrep System. The isolated RNA was used to synthesize cRNA which was then purified using non-Promega products. Total RNA including miRNA was isolated with a ReliaPrep miRNA Cell and Tissue MiniPrep System. (4980)

Notes: RNA collection from rat Achilles tendon was performed using a ReliaPrep™ RNA Tissue Miniprep System. RNA collection from cells was performed using a ReliaPrep™ RNA Cell Miniprep System. (4735)

Notes: HEK 293 cells were treated with thapsigargin and expression of target mRNAs was examined. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and reverse transcribed with the GoScript™ Reverse Transcription System followed by quantitation with the dye-based GoTaq® qPCR System. (4606)

Notes: Bodily fluids (buccal, circulatory blood, vaginal material, menstrual blood) were collected on sterile Cultiplast® rayon swabs. Total RNA was extracted from each sample with the ReliaPrep® RNA Cell Miniprep System and eluted in 50µl. Absence of detectable levels of human DNA (due to the on-column DNase treatment) was confirmed with a qPCR-based human forensic DNA quantitation test. Total RNA was converted to cDNA then amplified in a multiplex reaction with target-specific primer for analysis by capillary electrophoresis. (4601)

Notes: The effect of SMYD3 and ERK2 on hepatitis B RNA expression was examine in transfected Huh-7.5 human hepatoma cells. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and converted to cDNA with the GoScript™ Reverse Transcription System followed by dye-based qPCR analysis. The effect of SMYD3 and ERK2 on AP1-dependent and NF-κB-dependent gene expression in the cells was examined with the pGL4.44 [luc2P/AP1 RE/Hygro] and pGL4.32 [luc2P/NF-κB-RE/Hygro] Reporter vectors using the Dual-Luciferase® Reporter Assay System with a pRL-TK Renilla luciferase control. Reporter assays were read with a GloMax® Instrument. (4603)

Notes: RNA from three biological replicates of unstressed (static) and shear-stressed (flow) megakaryocytes were isolated using the ReliaPrep™ RNA Cell Miniprep System. (4736)

Notes: Serial 50µm thick coronal rat brain slices were cryosectioned and specific areas of interest were identified and micro-punched. Total RNA was extracted from the micro-punches with the ReliaPrep™ RNA Tissue Miniprep System. The RNA was used for dye-based, two-step RT-qPCR. (4593)

Notes: For global transcriptional analysis of mouse anterior segments, tissue was harvested from treated and control eyes 12 weeks after transplantation. Immediately after enucleation the trabecular meshwork was excised, preserved in RNAlater, and stored at −80 °C until further processing. RNA was then isolated using the ReliaPrep™ RNA Cell Miniprep System. (4739)

Notes: The authors used the ReliaPrep RNA Cell Miniprep System to isolate RNA from Drosophila wing imaginal discs prior to cDNA synthesis. (4727)

Notes: Total RNA was extracted using the ReliaPrep™ RNA Cell MiniPrep System for RT-qPCR. (4731)

Notes: ReliaPrep™ RNA Tissue Miniprep columns were used to clean up RNA extracted from C. elegans hermaphrodites prior to cDNA synthesis reactions. (4720)

Notes: LUHMES, a human embryonic mesencephalic cell line, was tested for the impact of overexpression of the PGAM5, a mitochondrial protein implicated in the molecular processes of Parkinson’s disease, with regard to 84 Parkinson’s associated genes. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and quality determined with a Agilent Bioanalyzer. The RNA was converted to cDNA and analyzed with the a Parkinson’s disease PCR array. The cDNA was also used in probe-based qPCR reactions. (4627)

Notes: Total RNA was isolated from U2OS treated with either SAHA or grown under starvation conditions and the levels of two transcripts were monitored by dye-based RT-qPCR. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System. (4628)

Notes: Total RNA was isolated from HTC-15 human colorectal carcinoma cells with the ReliaPrep™ RNA Cell Miniprep System. Isolated RNA was used in an RT-PCR method to amplify alternative splice variants of the target transcript. Once identified, a RT-qPCR experiment was designed to look for the major splice variants in normal cell lines derived from 15 human tissues (RNA isolation details not provided, assumed to be the same as HTC-15). The alternative splice variants were overexpressed in HTC-15 cells by transfection via FuGENE® HD Transfection Reagent and noted a decline in full-length expression. (4607)

Notes: C2C12 myotubes treated with BaF with and without amino acid deprivation were analyzed for gene expression by RT-qPCR. The RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and converted to cDNA with the ImProm-II™ Reverse Transcription System followed by dye-based qPCR. (4611)

Notes: Total RNA was isolated from mouse chondrocytes with the ReliaPrep™ RNA Cell Miniprep System and used for two-step, dye-based RT-qPCR analysis of two transcripts. (4631)

Notes: T47D and HB2 breast cancer cell lines were treated with doxorubicin and expressed in three target genes. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System, reverse transcribed with the GoScript™ Reverse Transcription System and analyzed with the dye-based GoTaq® qPCR System. (4612)

Notes: Total RNA was isolated from HeLa and A549 cells following downregulation of APE1 via siRNA transfection using the ReliaPrep™ RNA Cell Miniprep System. The levels of mRNA for a number of genes were monitored through dye-based RT-qPCR at 48, 72 and 96 hours post siRNA transfection. (4591)

Notes: Human pituitary cells were immortalized through expression of three genes. The immortalized cells were induced to secrete hormones and gene expression was monitored by RT-qPCR. Total RNA was extracted with the ReliaPrep™ RNA Cell Miniprep System. (4623)

Notes: Panc-1 pancreatc cancer cells were made resistant to a drug over time. The glycan structures on the surface of resistant and sensitive cells by passage over a lectin array, identifying a difference in fucosylation. Sensitive and resistant cell total RNA was isolated and examined by RT-qPCR for genes related to fucosylation. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System. (4624)

Notes: Primary rat beta-cells were prepared from islets and sorted by flow cytometry. Total RNA was prepared with the ReliaPrep™ RNA Cell Miniprep System prior to RNAseq. (4621)

Notes: Total RNA was extracted from grafted scales and surrounding epidermis of recipient fish with the ReliaPrep™ RNA Tissue Miniprep System. The RNA was used for dye-based two-step RT-qPCR to examine 12 transcript levels. (4630)