Notes: The MTT-based CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to measure the viability of serum-deprived prolactin-dependent Rat lymphoma Nb2 cells cultured in the presence of ovine prolactin and aurintricarboxylic acid (ATA). ATA was demonstrated to stimulate growth of Nb2 cells and protect against staurosporine-induced apoptosis. Promega’s Anti-ACTIVE™ MAPK pAb was used on Western blots to demonstrate that ATA stimulates phosphorylation of MAPK. (1702)

Notes: The CellTiter^® 96 Non-Radioactive Cell Proliferation Assay (MTT) was used to measure the proliferative response of quiescent Nb2 pre-B-cell lymphoma cells (40,000 cells/well) to control media, ovine prolactin and aurintricarboxylic acid (ATA). Nb2 cells proliferated in response to ATA, and ATA was found to activate ERK2. ATA at 250µM activated ERK2 within 4min as shown by blots with the Anti-ACTIVE^® MAPK pAb. (0464)

Notes: NGF-dependent pure sympathetic rat neurons from the superior cervical ganglion were cultured 2 days in various concentrations of NGF plus HGF, NGF plus anti-HGF or HGF alone. The surviving neurons were assayed with the CellTiter 96^® Non-Radioactive Cell Proliferation System. The authors use 50µl of Dye Solution to 500µl of media in a 24-well plate with a reaction time of 2 hours. The cells were lysed in 100µl of a 0.065N HCl/isopropanol solution rather than the recommended Solubilization/Stop Solution. (0142)

Notes: Murine macrophage cells (P388D1)  were pretreated with recombinant gamma interferon, cytochalasin D or mondansylcadaverine prior to infection with Bordetella bronchispetica and viability of the infected macrophages was determined using CellTiter 96^® Non-Radioactive Cell Proliferation Assay. (2540)

Notes: The CellTiter 96^® Non-Radioactive Cell Proliferation Assay was used to assess the growth factor requirements of murine erythroleukemia cells in culture. The cells were cultured for five days with various cytokines and growth factors prior to assay. Several of the IGF-1 independent clones analyzed by western blotting with the Anti-ACTIVE^® MAPK pAb were found to have a constitutive level of activated MAP Kinase. Study also shows serum is a very potent activator of MAPK in serum-starved cells. (0567)

Notes: The MTT-based CellTiter 96^® Non-Radioactive Cell Proliferation Assay was used to assess the effects of IGF-I, insulin or Stem Cell Factor (SCF) on growth of murine erythroleukemia cells. The effects of neutralizing antibodies against the growth factors were also tested. Promega’s Anti-ACTIVE^® MAPK pAb, with specificity for the active, dually phosphorylated form of MAPK was used for Western blotting to assay activation of mitogen-activated protein (MAP) kinases. Several of the IGF-1 independent clones analyzed by western blotting and found to have a constitutive level of activated MAP Kinase. Serum was shown to be a potent activator of MAPK in serum-starved cells. (2080)

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) was used to monitor the number of viable HeLa cells following exposure to an Anti-Fas antibody. HeLa cells stably transfected with usurpin lacking the DED domain were viable at higher doses of the anti-fas antibody than control cells. (0500)

Notes: The CellTiter^® 96 Non-Radioactive Cell Proliferation Assay was used to monitor proliferation of CID-9 mouse mammary epithelial cells transfected with a Cripto-1 expressing vector. (0612)

Notes: The broad-spectrum fluoroquinoline antibiotic, travafloxacin, was shown to suppress synthesis of various cytokines from cultured human monocytes. The CellTiter 96^® Non-Radioactive Cell Proliferation Assay (MTT) was used to determine the decrease in synthesis was not due to the toxic effect of the antibiotic on the monocytes. (0934)

Notes: The MTT-based CellTiter 96^® Non-Radioactive Cell Proliferation Assay was used to measure the effect of kinase inhibitors on the growth of NIH3T3 cells 2 days after UV irradiation. Kinase inhibitors significantly reduced UV-induced phosphorylation of Egr-1, transactivating ability and cell growth. (2088)

Notes: The CellTiter 96^® Non-radioactive Cell Proliferation assay was used to monitor the response of asynchronous and phase synchronized A375 cells to increasing amounts of endothelin-1. (0601)

Notes: Ldl-D or CHO cells were incubated with an anti-CD44 mAb or a isotype-matched control antibody and plated onto hyaluronan coated plates. Nonadherent cells were washed away and adherent cells quantified with the CellTiter 96^® Non-Radioactive Cell Proliferation Assay (MTT). Percentage adherent was determined by comparing washed wells to unwashed wells. (0374)

Notes: The authors used the CellTiter 96^® Non Radioactive Cell Proliferation Assay to study cell proliferation of IEC-6 and IEC-18 cells (rat intestinal epithelia). (0394)

Notes: Cytotoxicity of AT-61 for HepG2 cells an HEPAD38 cells (a HepG2 cell loine that produces HBV when grown in the absence of tetracycline) was assessed using the Cell Titer 96^® Non-Radioactive Cell Proliferation Assay. To assay for the inhibition of the tetracycline promoter activity in HepAD43 cells (produces β-galactosidase in the absence of tetracycline), β-galactosidase activity was determined using the Reporter Lysis Buffer from the β-Galactosidase Assay System. (2506)

Notes: HSG cells were cultured with laminin-derived peptides for 48 hours.  Cell proliferation was measured using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay. (2507)

Notes: Membrane co-factor protein (MCP) inhibits complement activation on host cells. Chinese Hamster Ovary cells were transfected with wildtype, reverse orientation, and mutant MCP lacking various domains. Cells bearing equivalent expression levels of the transfection product were tested in cytoprotection assays in a classical C pathway-mediated complement system using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay. The authors present data as (2484)

Notes: Primary cortical neurons isolated from day 18 mice were isolated and infected with Herpes Simplex Virus vectors expressing either the wildtype human presenilin-1 gene or a clinically significant mutant of the presenilin-1 gene. Cell viability assays of the infected cells were performed using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay to determine whether cell survival was affected. (2536)

Notes: The CAN gene product was expressed from a tetracycline inducible promoter in U937T cells. Induction of the protein inhibited cell growth, and uninduced cells were indistinguishable from untransfected control cells. Co-expression of Bcl-XL could not rescue the cells. Cell proliferation was monitored every day for four days using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay. Inhibition of cell proliferation was directly proportional to the amount of the CAN gene product expressed. (1420)

Notes: TNF-α cytotoxicity assays on WEHI-13VAR cells were carried out using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay. (2553)

Notes: Rat sympathetic neurons from the superior cervical ganglion of postnatal day 1 rats were used for survival assays in NGF-withdrawal experiments and p75 activation experiments. Cell survival was determined using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay. (2535)

Notes: The authors used a TnT^® Coupled Reticulocyte Lysate System to transcribe and translate Src in vitro. Cell proliferation of NIH 3T3 cells that stably expressed either HA-RACK or vector only was assessed using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay. (2537)

Notes: The authors used the CellTiter 96^® Non-Radioactive Cell Proliferation Assay to determine cell viability of mouse macrophage-like cells prior to all experiments measuring gene expression and TNF-α production. (2478)

Notes: NIH 3T3 cells with inducible expression of RNase L were treated with IFN-a/b then expression of RNase L was induced. The cells were then challenged with encephalomyocarditis virus and vesicular stomatitis virus. Viable cells 18hr after virus challenge were determined by use of the CellTiter 96^® Proliferation Assay (MTT). The interferon/RNase L induction only protected the cells from the encephalomyocarditis virus. (0800)

Notes: The authors performed a concerted mutagenesis and constructed point-mutated hβc libraries (hβc is the common signal transducing β subunit of GM-CSF, IL-3, and IL-5 receptors).  Mouse pro-B BAF-B03 cells and mouse myeloid FDC-P1 cells were transiently transfected with high-titer retroviruses carrying the mutant hβc cDNAs.  Lines expressing different mutated cDNAs were assayed for mGM-CSF- or mIL3- dependent and independent cell proliferation using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay. (2479)

Notes: The CellTiter 96^® Non-Radioactive Cell Proliferation Assay was used to monitor the proliferation of untransfected Ba/F3 and c-Kit-expressing Ba/F3 pro-B-cells in response to IL-3. No significant difference was observed over 8 days. (0887)