Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay was used to assess metabolic function of neuronal cells after treatment with various concentrations of beta-amyloid. (2967)

Notes: The CellTiter 96^® AQ_ueous Assay (MTS/PMS) was used to analyze the IC₅₀ of a taxol derivative. The assay was performed on taxol-sensitive and taxol-resistant cells. The SKOV3 cell line is sensitive to taxol, and SKVLB, a derivative of SKOV3, is resistant to vinblastine. The cell line A-549 as well as its taxol-resistant derivative, AT12, were tested also. (1073)

Notes: Treatment of the human medulloblastoma MED283 cells with NGF causes a reduction is cell viability over a 72-hour period as judged by the CellTiter 96^® AQ_ueous Cell Proliferation Assay. The death was due to apoptosis. Neither the PD98059 inhibitor nor the MEK Inhibitor U0126 could prevent the apoptosis. The inhibitors (50µM and 10µM, respectively) were added to the media, which was changed every 12 hours during the 72-hour period. Cells with inhibitor and no NGF were not apoptotic, nor was there any noticeable decrease in cell viability. The data for the MEK Inhibitor U0126 Inhibitor is not shown. (1296)

Notes: T98G human glioblastoma cells were transfected with antisense oligonucleotides to JNK isoforms as well as control scrambled peptides. The proliferation of the cell in the absence or presence of the oligonucleotides were analyzed and compared. Proliferation was measured with the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS). (0035)

Notes: The CytoTox^® 96 Non-Radioactive Cytotoxicity Assay was used to determine the toxicity of two peptides, a C-terminal fragment of APP (CT 105), and Amyloid β protein (Aβ 1-42). Cells were treated with the peptides in the presence or absence of neuroprotective agents cholesterol, MK801, nifedipine, or verapamil. Studies were done in PC12 cells and in SK-N-SH human neuroblastoma cells. (2134)

Notes: Primary mouse cortical neurons derived from caspase-12 normal and knockout mice were tested for cytotoxicity to amyloid beta peptides, staurosporine or a media component. Cytotoxicity was analyzed with the CellTiter 96^® AQ_ueous Assay (MTS/PMS). (0642)

Notes: A rat islet cell line stably expressing rat placental lactogen was generated. The bioactivity of the secreted lactogen was measure with the Nb2 cell assay. The proliferation of the Nb2 cells was assayed with the CellTiter 96^® AQ_ueous Assay (MTS/PMS). (1160)

Notes: PC12 cells were transfected to express cyclooxygenase-2 (Cox-2). Cox-2 expression was inducible with IPTG. Cox-2 expressing cells, in the presence of IPTG or without, proliferated to nearly the same levels as control, vector only transfected cells in response to EGF. The proliferation was measured with the CellTiter 96^® AQ_ueous Assay (MTS/PMS). The Cox-2 expressing cells were less prone to apoptosis when cultured with NGF and the NGF is withdrawn. Six hours after withdrawal of the NGF, the Cox-2-producing cells contain near control levels of caspase-3 activity as judged by the CaspACE™ Assay System, Fluorometric. (0712)

Notes: FDC-P1 cells transfected with a mutant human growth factor receptor were challenged with a mutant human growth hormone, termed E8, designed to bind to the mutant receptor. The proliferation of the cells under these conditions was normalized to the cell proliferation in the presence of IL-3. Proliferation was measured with the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS). (0041)

Notes: The CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to examine dopamine-induced toxicity. SK-N-SH human neuroblastomas were cultured for 40 hours with and without a glutathione-depleting compound prior to addition of dopamine for 24hours. Other compounds like ascorbate, pyruvate and manganese were tested in combination with the dopamine treatment. (0336)

Notes: The effect of chloride channel blockers on LLC-PK1 cell viability was examined with the CellTiter 96^® AQ_ueous Assay (MTS/PMS). (0681)

Notes: The CellTiter 96^® AQ_ueous Assay (MTS/PMS) was used in conjunction with the FDCP-hMPL5 cells as a bioassay for thrombopoietin. The FDCP-hMPL5 cells are a derivative of the FDCP-2 cells transfected with mpl-5 cDNA making it responsive to thrombopoietin. The assay was used to test over twenty mutants. (0637)

Notes: The proliferative response of AGS cells to gastrin and pentagastrin was measured with the CellTiter 96® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS). (0042)

Notes: RAW 264.7 macrophages were challenged with wildtype and recombinant anthrax lethal factor in the presence of protective antigen. Cytotoxicity was compared to untreated cells. Viable cells were measured with the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS). (0039)

Notes: The SK-N-MC human neuroblastoma cell line was treated with a nitric oxide donor, DEA/NO, and a enzyme secreted into the media was measured. To insure that the DEA/NO treatment did not harm the cells, the cell viability was examined with the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS). (0037)

Notes: The CellTiter 96^® AQ_ueous Assay (MTS/PMS) was used to measure the proliferation of HMVEC, human dermal microvascular endothelial cells, in respone to glucose analogs or bFGF. (1096)

Notes: The CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to analyze the proliferation of G401 cells in response to midkine, a retinoic acid-inducible heparin binding protein. Proliferation was measured in the presence of various compounds including heparin and heparinase. (2491)

Notes: The CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to assess the cytotoxicity of the fish apoptosis-inducing protein to human HL-60 myeloblasts. (0036)

Notes: The CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to determine the proliferation rate of human TF-1 pre-leukemic B cells. The cells were tested with conditioned media of cells infected with an IL-13-producing adenovirus. Preincubation of the media with a neutralizing anti-IL-13 antibody prevented the proliferation. (0034)

Notes: Mouse spleen cells containing 4 x 10⁴ CD4⁺ T cells/well were cultured in 96-well plates in triplicate and assayed for proliferation in response to a synthetic peptide after 60 hours in culture. The CellTiter 96^® AQ_ueous Cell Proliferation Assay was used to monitor proliferation. (1362)

Notes: The CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay was used in Rat Liver Epithelial cells (RLEs). Response to treatment with TGF-β₁ was compared between normal RLE cell lines and RLEs transformed with Ha-ras (F22-ras cell line), or with v-Raf (F3611-T2 and F3611-TH cell lines). TGF-β₁ treatment dramatically inhibited growth of the wild type RLEs, but two of the three transformed lines showed only a modest decrease in proliferation. Cells were plated in 96 well dishes at 2x10⁵ cells per well in 100 µl total volume. (2133)

Notes: The authors investigated the mechanism by which sphingosine 1-phosphate-induced cell proliferation occurs. Human hepatoma cells, HTC4, were plated in 96-well plates, serum-starved for 4 hours and treated with or without sphingosine 1-phosphate in serum-free medium. Cell proliferation was assayed using the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay. (2509)

Notes: The response of HL-60 cells to a Bcl-2 binding molecule, HA14-1, was examined with the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS). The compound strongly induced cytotoxicity via apoptosis. (0040)

Notes: A culture of TRL 1215 cells was grown for 18-20 weeks in the presence of low levels (500nM) of arsenic to generate a chronic exposed cell line. These cells were tested versus the original cell line for cytotoxicity due to arsenic. The chronically exposed cells were at least a log less sensitive to the chemical. the cytotoxicity was determined with the CellTiter 96^® AQ_ueous Cell Proliferation Assay. (2546)

Notes: The CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to determine the adherence of normal and transfected U251 human glioblastoma cells to fibronectin. The U251 cells were transfected with an EGR-1 transcription factor-expressing construct. The EGR-1 expressing cells adhered to the matrix better than the normal cells or vector only cells. The binding was blocked by RGD-containing peptides. (0038)