Notes: RNasin® Ribonuclease Inhibitor was used in a in vitro transcription reaction to produce capped transcripts. The template DNA was then removed by RQ1 RNase-free DNase. (0328)

Notes: Genomic DNA was isolated from mouse brain with the Wizard^® Genomic DNA Purification Kit. The isolated DNA was used for PCR. The RNasin^® Ribonuclease Inhibitor was used to protect RNA in RT-PCR. (0372)

Notes: Before RT-PCR reactions, the RNA template was treated with RQ1 RNase-free DNase. (0776)

Notes: The authors used RQ1 RNase-Free DNase to remove contaminating genomic DNA from total RNA samples. Cu, Zn, and MN SOD mRNA sequences were amplified from hippocampal neuronal RNA using the Access RT-PCR System. (2218)

Notes: The authors co-translated mRNAs for beta 2 microglobulin and MHC class I proteins in Flexi^® Rabbit Reticulocyte Lysate System with Canine Pancreatic Microsomal Membranes (CMMs). They generated mRNAs using RiboMAX™ T7 Large Scale RNA Production System. Treated transcription reactions with RQ1 RNase-Free DNase I after transcription to remove template DNA. (0393)

Notes: The authors used the pCAT^® Basic Vector in their promoter studies and RQ1 RNase-free DNase and rhSP1 in their footprinting studies with Drosophila SL2 cells. Promega's pCAT Vectors have now been replaced by the next generation pCAT^® 3 Reporter Vectors. (0407)

Notes: The Access RT-PCR System was used to study expression of three ntn genes from TW3 cells grown in either 4-nitrotoluene, toluene or succinate. The pET-5a Vector (discontinued) was used to express the ntnC protein in E. coli strain BL21(DE3) pLysS. (0968)

Notes: The RNasin Ribonuclease Inhibitor was used to protect RNA transcripts during primer extension analysis and in vitro transcription of RNA probes. The resultant RNA probes were used for RNase protection assays. (1639)

Notes: Poly(A)+ RNA was isolated from RIN rat insulinoma cell total RNA with PolyATtract® mRNA Isolation System III. (1264)

Notes: Poly(A+) RNA was isolated from RIN rat insulinoma cell total RNA and was used for Northern blots. The authors used Promega's PolyATtract^® mRNA Isolation System and AMV Reverse Transcriptase in this study. (2107)

Notes: Reporter studies were performed in the neural cell lines, CG-4 and PC-12, and the non-neural cell lines, HeLa and NIH3T3, using constructs prepared in the pCAT^®-Basic Vector. The pCAT^® Vectors have been replaced with the next generation pCAT^®3 Vectors. (1027)

Notes: Poly(A+) RNA was isolated from total RNA extracted from T47D mammary carcinoma cell line using the PolyATtract^® mRNA Isolation System. The RNA was used for northern blots. RQ1 was used to digest genomic DNA away from total RNA. The pGEM®-T Vector was used to subclone PCR products generated by differential display. (1673)

Notes: The inhibitor was used to protect transcripts during a run-on transcription assay. (1638)

Notes: The GDNF E_max ImmunoAssay System was used to quantify the level of GDNF in the conditioned media of HeLa cells transduced with a recombinant adenovirus expressing GDNF. The RQ1 DNase was used to treat RNA sample to remove genomic DNA and the AMV reverse transcriptase was used for first strand cDNA synthesis. (1605)

Notes: The Wizard^® Plus Minipreps DNA Purification Resin was used to isolate apoptotic DNA from primary cultures of cerebellar granule cells. The cells were lysed with 7M Guanidine HCl, mixed with 1ml of resin, spun down, resuspended in wash solution, collected in a minicolumn, washed and eluted in water. The DNA was treated with RNase A and treated with the nucleic acid stain, SYBR Green, for quantitation versus a DNA standard. Two hundred nanograms of the isolated DNA were analyzed by agarose gel electrophoresis and ethidium bromide staining. The RQ1 RNase-Free DNase was used to treated total RNA samples prior to RT-PCR. (1217)

Notes: Paraffin-embedded tissues were deparaffinized and treated with proteinase K. The sections were fixed in paraformaldehyde and labeled with digoxigenin-labeled dUTP. Histochemical detection was accomplished with an AP-conjugated antibody and BCIP/NBT staining. As a positive control, a section was treated with RQ1 RNase-Free DNase prior to labeling with Promega's TdT and the labeled-dUTP. (0211)

Notes: Promega's RQ1 RNase-Free DNase, M-MLV Reverse Transcriptase and RNasin^® Ribonuclease Inhibitor were used in this study. (2020)