Notes: Infection progression remains poorly characterized in Chagas disease, a life-long infection caused by T. cruzi. To understand the development of the disease, a bioluminescent imaging system for live mice infected with NanoLuc-labeled transgenic T. cruzi was developed. Initial expression of NanoLuc luciferase was determined in TcCOL cells and cell lysates using the Nano-Glo Live Cell assay and the Nano-Glo Luciferase Assay System, respectively. Luminescence could be detected as early as 14 days post-infection and was focused in the abdomen. Further, luminescence remained detectable for up to 222 days post infection, while parasite number in blood decreased after 21 days to the limit of detection.  (5110)

Notes: cDNA synthesized from RNA obtained from a rat hybridoma that produced a monoclonal antibody specific to bovine PD-1 was amplified and cloned into the pGEMT®-T Easy Vector. Additionally the Wizard® Genomic DNA Purification Kit was used to purify DNA from peripheral blood mononuclear cells (PBMCs) obtained from genomic DNA was extracted from antibody-inoculated cattle. (4771)

Notes: The researchers in this study used Wizard^® Genomic DNA Purification Kit to extract DNA to be used in the next-generation sequencign of the I-Scel breakpoint in U2OS EJ-DR cells. (4891)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Chryseobacterium rigui. (4318)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Edwardsiella ictaluri fhuC. (4328)

Notes: The authors of this study were conducting a genome-wide analysis to look for genetic loci necessary for bacterial cell growth and survival. Genomic DNA was purified from V. cholerae prior to amplification and high-throughput sequencing on the Illumina MiSeq platform. (4528)

Notes: The authors isolated initial genomic DNA from V. cholerae transposon library using the Wizard Genomic DNA Purification Kit followed ultimately by NGS with an Illumina MiSeq Instrument. (4921)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Escherichia coli. (4329)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Clostridium perfringens. (4319)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Haemophilus influenzae. (4336)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Algiphilus aromaticivorans. (4297)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Algoriphagus jejuensis. (4298)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Anaerosalibacter bizertensis. (4308)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Arthrobacter cupressi. (4309)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Escherichia coli. (4332)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Halanaerobacter jeridensis. (4337)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Enterococcus faecium. (4338)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Babesia spp. (4310)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Faecalibacterium prausnitzii. (4333)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Bacillus amyloliquefaciens. (4312)

Notes: The authors used microarray analysis, bisulfite sequencing and pyrosequencing to examine a possible link between aberrant DNA methylation and abnormal human spermatogenesis and male infertility. They identified almost 600 genes that were differentially methylated in testis tissue of men with secretory male infertility. Genomic DNA used in the microarray analysis was extracted from testicular biopsies using the Wizard® Genomic DNA Purification Kit. For the bisulfite sequencing experiments, genomic DNA was bisulfite-modified, amplified, cloned using the pGEM®-T Easy Vector System, then sequenced. (4257)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Bacteroides stercoris. (4313)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Acinetobacter baumannii. (4296)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Flavobacterium psychrophilum. (4334)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Clostridium tunisiense TJ. (4291)