Notes: To amplify yeast mutarotase, S. cerevisiae was used, and E. coli strain JM109 (Promega Cat.# L2001) was used for plasmid propagation. Fungal mycelia were harvested by filtration, washed, frozen and ground under liquid nitrogen. Genomic DNA was extracted using the Wizard Genomic DNA Purification System (Promega Cat.# A1120). RNA for hybridization and RT-PCR was extracted from mycelia using the SV Total RNA Isolation System (Promega Cat.# Z3101) and plasmid DNA isolated using the PureYield(TM) Plasmid Midiprep System (Cat.# A2492). (3919)

Notes: To examine the methylation state of DNA of cultured cells, genomic DNA was purified using the Wizard® Genomic DNA Purification Kit. The extracted DNA was restriction enzyme digested and then treated with bisulfite. (3978)

Notes: The authors generated population data for unrelated Caucasian-Mestizos from Columbia using the PowerPlex^® 2.1 System and the GammaSTR^® Multiplex. DNA was isolated from whole blood using the Wizard^® Genomic DNA Purification Kit or ReadyAmp™ Genomic DNA Purification System, then amplified per the manufacturer's recommendations. Amplified fragments were detected using a Hitachi FMBIO^® II fluorescence imaging system. (3856)

Notes: The authors wanted to purify and characterize the capsular polysaccharide (CP) produced by Staphylococcus haemolyticus strain JCSC1435. S. haemolyticus strains grown in TSB cultures were harvested, lysed with lysozyme and lysostaphin and genomic DNA isolated using the Wizard® Genomic DNA Purification Kit. The DNA was then used in CP gene PCR. Total RNA was isolated from exponential, postexponential, and stationary phases of S. haemolyticus growth and used in RT-PCR using the Access RT-PCR System. (3977)

Notes: Because of concerns about the consumption of histamine in wine (which makes histamine more potent), the quantity of lactic acid bacteria (LAB), which can produce histamine during winemaking, was determined in 264 samples of red wine from 116 wineries. DNA was isolated from LAB strains grown in De Man Rogosa Sharpe (MRS) broth using the Wizard^® Genomic DNA Purification Kit. Glass beads were used to disrupt the cells, and a modified Wizard^® Genomic DNA Purification protocol that included PVP treatment was performed. The purified DNA was used in qPCR analysis. (3741)

Notes: In this study, the Wizard® Genomic DNA Purification Kit was used to extract DNA from diluted fecal samples. The extracted DNA was used in PCR with specific primers to detect viral sequences. PCR fragments were gel-purified using the SV Gel and PCR Clean-Up System prior to sequencing to confirm the Bocavirus DNA identity. (4221)

Notes: The authors investigated the effect of neonatal bisphenol A (BPA) exposure in rats on expression of estrogen receptor α (ERα) transcripts. Alternative ERα transcripts in preoptic area of treated and untreated rats were quantified using real-time RT-PCR. Reverse transcription was performed using 4µg of total RNA, 200pmol random primers and 300 units M-MLV Reverse Transcriptase. Real-time PCR was performed using SYBR^® Green I to quantify amplified products. To determine if the changes in BPA-induced ERα transcript expression were caused by DNA methylation, the methylation status of the five ERα promoters was examined by bisulfite modification. Genomic DNA was isolated from rat tissue using the Wizard^® Genomic DNA Purification Kit, denatured with NaOH, then treated with hydroquinone and sodium bisulfite. Prior to methylation-specific PCR, DNA was cleaned up using the Wizard^® DNA Purification Resin as directed by the manufacturer. PCR products were cleaned up again using the Wizard^® SV Gel and PCR Clean-Up System, then subjected to restriction enzyme digestion and agarose gel electrophoresis to reveal methylation-dependent sequence differences. (3911)

Notes: In this paper, the authors studied the common region of mod(mdg4) and its role in homolog conjunction during meiosis. Genomic DNA was isolated from adult Drosophila using the Wizard^® Genomic DNA Purification Kit and mutations identified by PCR and sequencing of the introns. (3576)

Notes: These authors examined how the retrovirus Moloney murine leukemia virus (MuLV) selected tRNA, which is used for primer binding and initiating reverse transcription, by mutating in the primer binding sites (PBS). At various time points, high-molecular-weight DNA was isolated from cells infected with MuLV using the Wizard^® Genomic DNA Purification Kit. The purified DNA was amplified using primers to the PBS, sequenced and analyzed by an alignment program. (3742)

Notes: These authors sought to determine whether helpers in cooperatively breeding species reproduced or if all offspring were from the breeders. They created 32 breeding groups and tested the possibilities of reproductive skew under various conditions. Genomic DNA from breeders and helpers was isolated from ethanol-preserved 1–2 mm² finclip samples or from whole offspring using the Wizard^® Genomic DNA Purification Kit. Purified DNA was resuspended in 50µl of DNA Rehydration Solution and analyzed by PCR using seven microsatellite primer pairs. (3679)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from mouse embryonic stem (ES) cells, embryonic bodies (EB) and adult tissues. The purified genomic DNA was subjected to methylation-sensitive restriction fingerprinting (MSRF; digestion with methylation-sensitive restriction enzymes followed by amplification of 100ng of digested DNA), or 2µg DNA treated with bisulfite. (3419)

Notes: Chromosomal DNA from Gram-positive Bacillus anthracis was isolated using the Wizard^® Genomic DNA Purification Kit. (3420)

Notes: To construct a genome library of the nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255, genomic DNA was isolated from batch cultures using the Wizard^® Genomic DNA Purification Kit. (3416)

Notes: To examine the role of aryl hydrocarbon receptor (AHR) in dibenzo[a,l]pyrene (DBP) tumor development, AHR-responsive and nonresponsive murine fetuses were treated in utero with DBP. Genomic DNA was isolated from lymphomas using the Wizard^® Genomic DNA Purification Kit. Two microliters of the purified DNA was PCR-amplified for analysis of the Ki-ras locus, where mutations are associated with tumor susceptibility. (3421)

Notes: The authors wanted to develop a more sensitive assay to detect codon 816 pathogenic variations in people diagnosed with systemic mastocytosis. The Wizard^® Genomic DNA Purification Kit was used to isolate DNA from peripheral blood and bone marrow aspirate samples. To extract DNA from 2–5 micron, paraffin-embedded samples of bone marrow trephine, skin, spleen or liver, the tissues were digested with Proteinase K for four days at 56°C prior to DNA purification using the Magnesil^® Genomic Fixed Tissue System. The isolated DNA was subjected to two assays: enriched sequencing of mutant alleles (ESMA) after BsmAI restriction enzyme digestion, and allele-specific competitive blocker PCR (ACB-PCR). (3575)

Notes: To examine if the depletion of Drosophila transcription termination factor (DmTTF) after RNAi treatment could reduce the gene copy number, genomic DNA was isolated from RNAi-treated and untreated Drosophila embryonic D.Mel-2 cells using the Wizard^® Genomic DNA Purification Kit. The mitochondrial ND3 gene and the nuclear H2B histone gene were used as probes for the Xho I-digested, Southern-blotted genomic DNA to compare the treatment groups. The Wizard^® SV Gel and PCR Clean-Up System was used to clean up the PCR-amplified probes. (3418)

Notes: The authors wanted to learn more about the proteins that comprise the Tetrahymena
phagosome proteome. Proteins were isolated from phagosome preparations, denatured by adding 5mM dithiothreitol and heating to 60°C for 1 hour. The samples were cooled to room temperature and alkylated by incubation with 10mM iodoacetamide for 1 hour protected from light. Sequencing Grade Modified Trypsin, at a 1:20 concentration (wt/wt) with an equal volume of 50mM ammonium bicarbonate, was added to the sample and incubated overnight at 37°C. Each sample was analyzed by two-dimensional liquid chromatography–tandem mass spectrometry (LC-MS/MS). Whole-cell Tetrahymena DNA was isolated using the Wizard^® Genomic DNA Purification Kit protocol for isolating genomic DNA from plant tissue omitting the use of liquid nitrogen with mortar and pestle. The isolated DNA was then used for restriction digestion, ligation and sequencing. (3680)

Notes: These authors studied the effect of transgenic Bacillus thuringiensis (Bt) corn feed containing the Cry1A gene on broiler chicken performance. They also analyzed the degradation of the Cry1A(b) transgene in the digestive tract of the chickens. Blood and samples from cecum, jejunum, gizzard, and crop were collected from ten broilers per treatment (fed Bt corn versus near isogenic corn). Genomic DNA was isolated from blood samples and the intestinal contents of the crop and gizzard using the Wizard^® Genomic DNA Purification Kit. Fifty nanograms of purified DNA were then used in PCR analysis. (3678)

Notes: In this study, the Wizard^® Genomic DNA Purification Kit was used to isolate chromosomal DNA from Salmonella enterica serovar Typhi. The isolated DNA was used in multiplex PCR to determine the presence or absence of certain DNA regions in various clinical isolates.  (3127)

Notes: Researchers used the Wizard^® Genomic DNA Purification Kit to isolate genomic DNA from non-Agrobacterium field bacteria samples. The isolated genomic DNA was used in PCR to amplify regions of the 16S rRNA gene. The PCR products were cleaned up using the Wizard^® SV Gel and PCR Clean-Up System and used in sequencing reactions. The paper also mentions the use of the Wizard^® Magnetic DNA Purification System for Food to isolate Agrobacterium radiobacter from cucumber root mats grown in the lab. (3190)

Notes: The Wizard^® Genomic DNA Purification Kit was used to purify genomic DNA from Burkholderia thailandensis and Burkholderia pseudomallei.  The genomic DNA was used in PCR to amplify the arabinose assimilation operon.  (3104)

Notes: The Wizard^® Genomic DNA Purification Kit was used to purify genomic DNA from Yersinia pestis Kimberley53 strain.  The isolated genomic DNA was digested with restriction endonucleases and then used in PCR amplifications. The amplified products were sequenced to verify the presence of a transposon insertion site.  (3103)

Notes: These authors assessed the risk of transmitting genetic defects to children after intracytoplasmic sperm injection (ICSI) for treatment of male factor infertility. Genomic DNA was isolated from blood of both parents and children using the Wizard® Genomic DNA Purification Kit. Multiplex amplification of 22 sequence tagged sites on the Y chromosome was then performed on 123 samples using the Y Chromosome Deletion Detection System, Version 1.1 and a prototype Multiplex E from Promega. (3117)

Notes: The Wizard® Genomic DNA Purification Kit was used to purify genomic DNA from Bacillus cereus.  The isolated DNA was used in PCR to create probes for Northern blotting Bacillus cereus total RNA to detect rsbV and orf4 gene transcripts.  (3100)

Notes: Allele frequencies for a Mestizo population in Columbia were determined with the PowerPlex^® 16 System. DNA was purified using the Wizard^® Genomic DNA Purification Kit.  (3003)