Notes: NIH 3T3 cells were transfected using Superfect (Qiagen) with Promega's pGL3-Control Vector and a marker for antibiotic resistance at a 5:1 ratio. Transfected cells were visualized after in situ addition of 500μM Beetle Luciferin in 10% FCS medium.  The transfected cells were used to show that light from a luciferase reaction can be used to create targeted cytotoxicity in the presence of the photosensitizer Rose Bengal (RB). This application has specific relevance as a  tumor treatment by photodynamic therapy. (2671)

Notes: Total RNA was isolated from Arabidopsis tissues using the RNAgents^® Total RNA Isolation System. The RNA was further processed into the poly(A) fraction using the PolyATtract^® mRNA Isolation System. The isolated RNA was used in Northern blot analysis. Blots were probed with a ³²P-labeled RNA probe generated from a cDNA cloned into the pGEM^®-T Easy Vector using the T7 Riboprobe^® in vitro Transcription System. Bioluminescence from transgenic plants containing a firefly luciferase reporter was visualized by spraying the plants with a solution of 5mM Beetle Luciferin, Potassium Salt in 0.1% Triton^® X-100. (2570)

Notes: NAC1 cDNA fragments encoding different portions of NAC1 were cloned into the plasmid pGal and cobombarded into onion skin cells with the reporter gene plasmid pTALuc. Inner onion peels were placed on a filter soaked with liquid MS medium and bombarded with 2µg of each plasmid. After bombardment, plates containing the bombarded tissues were incubated in a growth chamber for 16 hours and then sprayed with 50mM Beetle Luciferin. Luciferase activity was monitored after 10 minutes incubation in the dark. Luciferase activities were measured by a highly sensitive camera CCS system-ST138S. (2137)

Notes: Beetle Luciferin was used to look at E2F-responsive luciferase expression in a U2OS cell system. A synthetic 6xE2F-Luc reporter was microinjected into the nuclei of the cells. Twenty-four hours later, 1mM beetle luciferin was added to the medium, and emitted photons were acquired using a CCD camera. (2143)