Notes: This Assay Guidance Manual provides a resource to scientists optimizing assays used in drug discovery and development. The cytotoxicity chapter outlines several assays to detect dead cells, including LDH-Glo™, CytoTox-Glo™ and CellTox™ Green Cytotoxicity Assays, CellTiter-Glo® Luminescent Cell Viability Assay, CytoTox 96® Non-Radioactive Cytotoxicity Assay and CytoTox-ONE™ Homogeneous Membrane Integrity Assay. (5198)

Notes: Lactate dehydrogenase (LDH) release was used to monitor toxicity of compounds mediating apoA-I transcription on HepG2 cells. A luciferase reporter gene assay with renilla luciferase control was also used to evaluate the transactivation of PPARalpha. (5186)