Notes: To examine which amino acids of ArsA may be important for metalloid binding and transport, substitution mutations were introduced in the arsA gene using the Altered Sites^® II in vitro Mutagenesis System. Plasmids were purified using the Wizard^® Plus Minipreps DNA Purification System and subjected to restriction enzyme digestion. (3516)

Notes: To test the feasibility of the Mycobacterium bovis Ag85B gene as a DNA vaccine, the gene was amplified by PCR, introducing EcoRI and XbaI restriction endonucleases onto the primers, digested with the restriction enzymes and ligated into the pCI Mammalian Expression Vector. After transformation, the constructs were isolated using the Wizard^® Plus Minipreps DNA Purification System and analyzed using endonuclease digestion and DNA sequencing. To confirm production of a maltose binding protein (MBP)-Ag85B fusion protein in E. coli, the cell lysate was analyzed by Western blot, stained with a rabbit anti-MBP serum and secondary antibody Anti-Rabbit IgG (Fc), AP Conjugate (1:10,000 dilution) and developed with BCIP/NBT. BALB/c mice pretreated with 10mM cardiotoxin prior to intramuscular DNA immunization using construct pCI-Ag85B, administered at days 0, 15, 30, and 45. The immunization course was 50µl of DNA at a concentration of 1µg/µl in PBS with each animal receiving a total of 100µg of plasmid DNA. Antibody response was assessed at 15 days with an ELISA assay and cytokine presence tested two weeks after immunization using splenocytes for ELISA assays and ICC. (3552)

Notes: cDNA fragments from the Nogo gene were amplified from genomic DNA and cloned into the pGEM^®-T vector. The Wizard^® Plus Mini- and Midiprep kits were used to purify the plasmids from bacterial cells. (2658)

Notes: T4 Polynucleotide Kinase was used to phosphorylate primers before ligating them into a phagemid vector to allow cloning of Sfi I-restricted DNA fragments. Phagemid was prepared from XL1-Blue cells using the Wizard® Minipreps DNA Purification System. Inserts were PCR amplified from the phagemid and purified using the Wizard® PCR Preps DNA Purification System. In separate experiments, the cDNA for the melanin concentrating hormone receptor 1 cDNA was cloned from total melanocyte RNA using MMLV Reverse Transcriptase. In vitro translation of the cDNA was performed using the TnT T7 Coupled Reticulocyte Lysate System and Canine Pancreatic Microsomal Membranes. (2602)

Notes: Genomic DNA was extracted from 100 Drosophila melanogaster and 100 Drosophila virilis using the Wizard^® Genomic DNA Purification Kit.  The isolated genomic DNA was used for PCR amplification and direct sequencing.  RT-PCR was also performed in the presence of RNasin^® Ribonuclease Inhibitor and cloned amplimers were purified with the Wizard^® Plus Minipreps DNA Purification System prior to fluorescent sequencing. (2505)

Notes: The Wizard^® Plus Minipreps DNA Purification System was used to isolate apoptotic DNA from RPMI 8226 cells. (0820)

Notes: Plasmid DNA was purified with the Wizard® Plus Minipreps DNA Purification System. DNA sequencing was performed with the fmol® DNA Sequencing System. (0004)

Notes: Plasmids were purified with either the Wizard^® Plus Minipreps DNA Purification System or the Wizard^® Plus Midipreps DNA Purifications System. PCR products were purified with the Wizard^® PCR Preps System. (0751)

Notes: The pGEM® -T Easy Vector Systems, Wizard® PCR Preps DNA Purification System and Wizard® Plus Minipreps DNA Purification Systems were used in this study. (1959)

Notes: The Wizard® Plus Minipreps DNA Purification System was used to rescue an expression plasmid from Dictyostelium discoideum. The rescued plasmid was sequenced to confirm that no mutations of the DNA had occurred within the organism. (1124)

Notes: The Transfectam® Reagent was used to transiently transfect NIH 3T3, COS-7 and HeLa cells. A lot of detail is provided for the transfection method. Wizard® Plus Miniprep DNA Purification System and Wizard® PCR Preps DNA Purification System were used for plasmid purification and PCR product purification from low melting point agarose, respectively. (0226)

Notes: The Anti-BDNF pAb and the Anti-NT-3 pAb were used to demonstrate BDNF and NT-3 expression, respectively, in retrovirally transformed human Schwann cells. The immunocytochemistry was observed with anti-chicken IgY-FITC conjugate. The authors also used the Wizard^® Plus Minipreps DNA Purification System for plasmid isolation and Promega restriction enzymes for construction of the retroviral vectors. (0446)

Notes: The authors used the Wizard^® Plus Minipreps DNA Purification Resin to isolate genomic DNA from apoptotic cells. The DNA was run on a 1% agarose gel to detect internucleosomal degradation. (0408)

Notes: Plasmid DNA was purified with the Wizard® Plus Miniprep DNA Purification System and further purified for microinjection. Transgenic zebrafish were identified by PCR using Taq DNA Polymerase. PCR products were resolved on gels with the 100bp DNA Ladder as a size marker. (1183)

Notes: All plasmids were isolated with the Wizard^® Plus Minipreps DNA Purification System and eluted with sterile distilled water. The isolated DNA was used for microinjection into X. laevis oocytes for expression. (0437)

Notes: The authors use the Reverse Transcription System, Wizard^® PCR Preps DNA Purification Systems, Wizard^® Plus Minipreps DNA Purification System and the pGEM^®-T Vector System in their studies. (0445)

Notes: The SILVER SEQUENCE™ DNA Sequencing System, Rabbit Reticulocyte Lysate System, Wizard^® Plus Minipreps DNA Purification System and Taq DNA polymerase were used in this study. (1473)

Notes: DNA was isolated with the Wizard^® Plus DNA Purification System and ethanol-precipitated with ammonium acetate prior to transfection. The DNA was used for calcium phosphate transfection of PC12 and HepG2 cells. (0283)