Notes: To better understand tumor progression in mice carrying the Min allele of Adenomatous polyposis coli (Apc), a longer lived cross was generated and studied. Intestinal tumors and adjacent normal tissue were microdissected, frozen in liquid nitrogen and genomic DNA isolated using the Magnesil^® Genomic, Fixed Tissue System. The purified DNA was then used for microsatellite instability (MSI) analysis. (4067)

Notes: In this study of the frequency of human papilloma virus (HPV) in patients with oral squamous cell carcinoma (OSCC), the MagneSil^® Genomic, Fixed Tissue System was used to isolate DNA from formalin-fixed paraffin-embedded tissue samples from primary tumors and matched samples. Five microliters of genomic DNA was amplified using PCR Master Mix and primers for both a 110 bp fragment of human ß-globin gene and HPV genotype. After PCR, the product was analyzed on an 8% nondenaturing polyacrylamide gel and stained with AgNO₃. (3938)

Notes: To compare the parathyroid hormone (PTH) gene from a human parathyroid adenoma sample to DNA from the same individual’s peripheral blood leukocytes, the tumor genomic DNA was purified from fixed, paraffin-embedded tissue using the MagneSil^® Genomic, Fixed Tissue System. The isolated genomic DNA was then amplified using PCR primers for three exons and sequenced for analysis. (3557)

Notes: The authors wanted to develop a more sensitive assay to detect codon 816 pathogenic variations in people diagnosed with systemic mastocytosis. The Wizard^® Genomic DNA Purification Kit was used to isolate DNA from peripheral blood and bone marrow aspirate samples. To extract DNA from 2–5 micron, paraffin-embedded samples of bone marrow trephine, skin, spleen or liver, the tissues were digested with Proteinase K for four days at 56°C prior to DNA purification using the Magnesil^® Genomic Fixed Tissue System. The isolated DNA was subjected to two assays: enriched sequencing of mutant alleles (ESMA) after BsmAI restriction enzyme digestion, and allele-specific competitive blocker PCR (ACB-PCR). (3575)

Notes: In this study, coding sequence abnormalities in desert hedgehog (DHH), a gene involved in male sex differentiation, were investigated. Genomic DNA was isolated from paraffin-embedded gonadal tissue from individuals with mixed gonadal dysgenesis and from three controls using the MagneSil^® Genomic, Fixed Tissue System. The purified DNA was then analyzed by exon-specific PCR, single-stranded conformation polymorphism (SSCP) analysis, and sequencing. (3447)

Notes: This paper describes use of the MagneSil® Genomic, Fixed Tissue System for purification of genomic DNA from paraffin-embedded gonadal tissues. The isolated genomic DNA was used in PCR to amplify a sequence from Desert hedgehog (DHH) Gene. The PCR products were used in sequencing reactions. (3179)