Notes: Poly(A)⁺ RNA was purified from total RNA by the PolyATtract^® mRNA Isolation System. PCR products were purified by agarose gel electrophoresis and were cloned into pGEM^®-T and pGEM^®-T Easy Vector. Genes were obtained by RT-PCR using the Access RT-PCR System. (1097)

Notes: Total RNA was isolated from a virulent strain of Salmonella enterica, SL1344 using the SV Total RNA Isolation System. The Access RT-PCR System was used to characterize the transcriptional organization of the prg operon. The pgrH gene was amplified by RT-PCR and cloned into the pGEM^®-T vector (2305)

Notes: Poly A+ RNA was isolated from infected cynomolgus monkey plasma and used for RT-PCR with the Access RT-PCR System. (0396)

Notes: The Access RT-PCR System was used to amplify Vα₁₀Jα₂₄ mRNA of the TCR alpha chain and the resulting product was cloned with the pGEM^®-T Vector System. (0734)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate RNA from LPS-stimulated, bovine leukemia virus-infected peripheral blood mononuclear cells. The RNA was treated with DNase and used for RT-PCR with the Access RT-PCR System. One fiftieth of the reaction was used for nested PCR. (0855)

Notes: The Access RT-PCR System was used to amplify messages for spinophilin and neurabin from rat brain total RNA. The products were used to make probes to screen a cDNA library. (0720)

Notes: Total RNA was isolated from the cortex and cerebellum of adult and developing rat brains with the RNAgents^® Total RNA Isolation System. RT-PCR was performed on a variety of samples with a variety of primers to assay alternative splicing. The Access RT-PCR System was used for all RT-PCR. (0764)

Notes: The authors used the Access RT-PCR System to perform quantitative RT-PCR from total RNA. (2239)

Notes: Total RNA was extracted from HEL-5J20 and used to generate a full length cDNA (576bp) of the calcium- and integrin-binding protein using the Access RT-PCR System. The subsequent product was purified with the Wizard^® PCR Preps System, digested with restriction enzymes and cloned into a GST-fusion expression vector. (0214)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from human skin biopsies. Two hundred nanograms of the isolated RNA was used for RT-PCR amplification with the Access RT-PCR System. For added sensitivity, nested PCR was performed as well. (0108)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from RAW 264.7 macrophages treated for various times with endotoxin. The isolated RNA was used for RT-PCR analysis of High Mobility Group-1 gene expression as well as β-actin. RT-PCR was performed with the Access RT-PCR System. (0198)

Notes: The p53 cDNAs expressed from thymic lymphoma cells were amplified with the Access RT-PCR System and the resulting products were cloned with the pGEM^®-T Vector System. No details of the reaction or whether or not total RNA was used were provided. (0812)

Notes: Synthesis of the mod-D1 cDNA and PCR amplification was performed using the Access RT-PCR System. (0744)

Notes: Total RNA was isolated from Pseudomonas putida using the SV Total RNA Isolation System. RT-PCR using the Access RT-PCR System allowed the authors to characterize the transcriptional organization of several genes involved in naphthalene degradation. (2307)

Notes: The Access RT-PCR System was used to analyze collagen gene expression of primary fibroblasts treated with various agents. The messages were amplified from 100, 200 and 400ng of total RNA. (0928)

Notes: The SV Total RNA Isolation System was used to extract total RNA from 106 SK-Mel2 human melanoma cells. The isolated RNA was used for RT-PCR with the Access RT-PCR System. The results were used for semi-quantitative analysis. (0959)

Notes: The initial work was performed with a single-tube RT-PCR system from a company in which both the reverse transcriptase and polymerase were mixed together and thus could not be separated. Consequently, the authors could not produce the important no-reverse transcriptase control. To do such a control, the authors used the Access RT-PCR System, which allows such control, since both the Tfl DNA Polymerase and AMV Reverse Transcriptase are added separately to the reaction. (0925)

Notes: The Access RT-PCR System was used for analytical RT-PCR for a variety of targets. Amplification was performed within the linear range of PCR product formation and normalized to the beta-tubulin signal or rpS6 signal. (1169)

Notes: Genomic DNA was purified from blood or cultured fibroblasts, by means of the Wizard^® Genomic DNA Purification Kit. Total RNA was isolated from cultured fibroblasts by use of the SV Total RNA Isolation System, and it was amplified by Access RT-PCR System by use of exonic primers. An expression vector containing an S-tag attached to the 5´ end of the FALDH cDNA was constructed using pCI-neo Mammalian Expression Vector. (0482)

Notes: The Access RT-PCR System was used to determine HPRT (hypoxanthine-guanine phosphoribosyltransferase) and iNOS (induced nitric oxide synthase) mRNA in salivary gland lysate of P. papatasi. The Griess Reagent System was used to measure the nitrite accumulation in macrophage culture media. (0191)

Notes: cDNAs for the receptor genes were obtained by RT-PCR using the Access RT-PCR System. (0569)

Notes: The authors used RQ1 RNase-Free DNase to remove contaminating genomic DNA from total RNA samples. Cu, Zn, and MN SOD mRNA sequences were amplified from hippocampal neuronal RNA using the Access RT-PCR System. (2218)

Notes: The Access RT-PCR System was used to determine the presence of hepatitis C virus RNA in chimpanzee serum. (1465)

Notes: The Access RT-PCR System was used to compare the abundance of GnT-IV mRNA in various bovine tissues. (0694)

Notes: The authors compared a commercially available enterovirus-specific RT-PCR kit with a kit of their own design. Their 'in-house' RT-PCR system used the Access RT-PCR System to amplify enterovirus sequences from cerebral spinal fluid extracts followed by nested-PCR for final amplification of the target. Both the commercial system and the 'in-house' system were adequate for amplification of enterovirus sequences from CSF of infected individuals. Other, more time-consuming assays were performed to verify the results from the RT-PCR tests. (0545)