Notes: Quantitative RT-PCR was carried out using 1µg total RNA from Arabidopsis using Access RT-PCR System. Samples were taken during the PCR reaction after 5, 10, 15, and 25 cycles and loaded onto agarose gels. Gels were treated for Southern blot hybridization, and filters were hybridized using the AtMYB2 cDNA. Linearity of signal strength was verified using phosphorimaging system quantifications. (1048)

Notes: The Access RT-PCR System was used to amplify two portions of the CHD1 gene product from ostrich poly A+ RNA. The amplification products were gel purified and sequenced. The sequence obtained was compared to that of the human and murine CHD1 and Chicken CHD1Z and CHD1W sequences. (1175)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from normal skin, psoriatic skin, H-128 small cell lung carcinoma cells and peripheral blood mononuclear cells. The isolated RNA was used for RT-PCR performed with the Access RT-PCR System. (0107)

Notes: The Access RT-PCR System was used to amplify the lactate dehydrogenase gene from a variety of Antarctic notothenioid fish. (1154)

Notes: The Access RT-PCR System was used to assess IL-8 (interleukin-8) and ENA-78 (epithelial cell-derived neutrophil-activating peptide) mRNA in M. hominis-infected and untreated A549 cells. (0894)

Notes: The PolyATtract^® System 1000 was used to isolate polyA+ RNA directly from peripheral blood leukocytes. A 30µl preparation of mRNA was isolated from 1.2 x10⁹ cells. Three microliters of the isolated RNA was used for RT-PCR amplification of the p53 message with the Access RT-PCR System. (1656)

Notes: The Access RT-PCR System was used to study expression of three ntn genes from TW3 cells grown in either 4-nitrotoluene, toluene or succinate. The pET-5a Vector (discontinued) was used to express the ntnC protein in E. coli strain BL21(DE3) pLysS. (0968)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from mouse fibroblasts. The isolated RNA was used for RT-PCR analysis with the Access RT-PCR System. (0474)

Notes: The Access RT-PCR System was used for a semi-quantitative assessment of the quantity of Cu/Zn superoxide dismutase (Cu/Zn SOD) Mn superoxide dismutase (Mn SOD) mRNA levels. Primary embryonic hippocampal neurons were treated with Abeta(25-35) for zero to 25 hours. The level of the Cu/Zn SOD and Mn SOD mRNA was related as percent of control, untreated cultures. The PCR portion of the reaction was performed for 22 cycles to insure that the reaction was in the linear range. (0106)

Notes: The Access RT-PCR System was used to assess expression of the CIITA gene in fibroblast L and splenic B cells. The 700bp transcript could be detected with as little as 40ng of poly(A)+ RNA from the splenic B cells but undetectable in as much as 5µg of poly (A)+ RNA from the L cells. Both sources of RNA were readily amplified for a 510bp β-actin message from 5µg down to 40ng. (0657)

Notes: The Access RT-PCR System was used to study expression of Bcl-2, A1, Bax and Bad genes in HUVE (human umbilical vein endothelial) cells treated with VEGF (vascular endothelial growth factor). The technique of 'real-time' quantitative RT-PCR was employed. (1150)

Notes: The authors used Promega's AMV Primer Extension System and Access RT-PCR System for this study. (2216)

Notes: Promega's Access RT-PCR system, Taq DNA Polymerase and PolyATtract^® mRNA Isolation System were used in this study. (1991)

Notes: The Access RT-PCR System was used in this study. (1989)

Notes: The Access RT-PCR System was used to amplify the FasL message from total RNA isolated from mice injected with a recombinant adenovirus. The Access RT-PCR System was used as a means to monitor the success of a gene therapy system. The products were analyzed by EtBr-staining and Southern blotting. (0088)

Notes: The Access RT-PCR System was used in this study. (1994)

Notes: The Access RT-PCR System was used in this study. (1988)

Notes: The Access RT-PCR System was used to monitor the expression of the type II iodothyronine deiodinase in primary rat astrocyte cultures following forskolin treatment. (1592)

Notes: The Access RT-PCR System was used in this study. (1998)

Notes: The Access RT-PCR System was used to demonstrate expression of the transfected cDNAs in cells. The CellTiter 96^® Non-Radioactive Cell Proliferation Assay (MTT) was used to monitor cell survival after diphtheria toxin exposure. (0698)

Notes: The authors used Promega's Access RT-PCR System in this study. (2220)

Notes: The Access RT-PCR System was used to amplify both lacZ and bioA in duplex. Thirty-five nanograms of bacterial RNA was amplified for 25 cycles and the amount of lacZ message was normalized to the level of bioA. (0190)

Notes: Real-time quantitative RT-PCR was performed using the Access RT-PCR System. (1102)

Notes: Promega's Access RT-PCR System was used in this study. (1997)