Notes: M-MLV Reverse Transcriptase (Cat.# M3681) was used with Zika virus primers in real-time RT-PCR. (4652)

Notes: Researchers used M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant, and random hexamers to reverse transcribe total RNA from various rat tissues. The cDNA was then used in real-time PCR reactions with SYBR Green dye and primers specific to Krim-1 or GAPDH sequences.  For reverse transcription, 200 units of M-MLV were used in each 25μl reaction.  Data from real-time PCR was normalized to GAPDH levels and expressed as relative expression levels in various tissues.  (2792)

Notes: Total and poly(A)+ RNA from treated and untreated 8701-BC breast cancer tumor cells were treated with RQ1 RNase-free DNase. The RNA samples were reverse transcribed using the M-MLV RNase H– point mutant reverse transcriptase and random primers to create cDNA used in downstream analyses. The cDNA from the reactions were used in PCR, differential display PCR, and semi-quantitative multiplex PCR reactions. In differential display PCR studies, the authors analyzed differentially displayed bands by staining 6% polyacrylamide gels with the SILVER SEQUENCE™ Staining Reagents. Differentially displayed bands were re-amplified and cloned using the pGEM®-T Easy Vector and high efficiency JM109 competent cells. (3020)

Notes: Single cell RT-PCR was performed using M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant, and the extracted cytosol of sympathetic neurons isolated from 17-day-old or 45-day-old rats. An oligo(dT) primer was used in the reverse transcription reactions. The resultant cDNA was used in PCR with KCNQ2-, KCNQ3-, KCNQ4-, and KCNQ5-specific, potassium channel subunit gene primers.  (3024)