Notes: Wizard® SV Gel and PCR Clean-Up System was used to clean up genomic DNA isolated from parasitic nematodes isolated from a variety of animals. Species identification of each nematode specimen was determined via PCR amplification of specific nuclear DNA followed by purification of the amplified product using Wizard® PCR Preps DNA Purification System before sequencing using BigDye chemistry. Wizard® SV Gel and PCR Clean-Up System was also used to prepare amplicons generated by long-PCR of mt genomes from the nematodes before NGS sequencing. Results from NGS were confirmed using PCR-based sequencing of short mt DNA tracts. Short mtDNA regions were amplified by conventional PCR. Amplicons were purified using Wizard® PCR Preps DNA Purification System before sequencing using BigDye chemistry. (4533)

Notes: The authors studied the relationship between transient receptor potential canonical (TRPC) channels and Ca²⁺ elevation in the cannabinoid-2 receptor-expressing human peripheral blood-acute lymphoid leukemia (HPB-ALL) human T cell line. Total RNA from HPB-ALL cells was subjected to RT-PCR and the bands for TRPC1 were excised from a 1.2% NuSieve 3:1 agarose gel, purified using the Wizard® PCR Preps DNA Purification System and sequenced. Using 20nM synthesized siRNA specific for TRPC1 and a nonsilencing control sequence, 2.5 × 10⁵ HPB-ALL cells/ml (a human T cell line) were transiently transfected for 48 hours using the CodeBreaker™ siRNA Transfection Reagent. After this 48-hour incubation, the siRNA-treated cells were either used for calcium determination or harvested and washed, and the RNA was isolated using the SV Total RNA Isolation System. The RNA was used for quantitative real-time PCR. (3316)

Notes: Three terminal repeat sequences (TR) from Kaposi's sarcoma-associated herpesvirus (KSHV) were PCR amplified and then purified using the Wizard^® PCR Preps DNA Purification System. The PCR products were 386, 424, and 307 base pairs in size.  These TR products were used in PARP1-DNA ELISA assays.  The authors also used the Wizard^® SV Genomic DNA Purification System to isolate DNA from a BC3 cell line infected with KSHV. The isolated DNA was used in real-time PCR to assess KSHV copy number in cells. (3232)

Notes: T4 Polynucleotide Kinase was used to phosphorylate primers before ligating them into a phagemid vector to allow cloning of Sfi I-restricted DNA fragments. Phagemid was prepared from XL1-Blue cells using the Wizard® Minipreps DNA Purification System. Inserts were PCR amplified from the phagemid and purified using the Wizard® PCR Preps DNA Purification System. In separate experiments, the cDNA for the melanin concentrating hormone receptor 1 cDNA was cloned from total melanocyte RNA using MMLV Reverse Transcriptase. In vitro translation of the cDNA was performed using the TnT T7 Coupled Reticulocyte Lysate System and Canine Pancreatic Microsomal Membranes. (2602)

Notes: Chromosomal DNA was isolated from V. cholerae with the Wizard® Genomic DNA Purification Kit prior to PCR.  The PCR products were directly purified with the Wizard® PCR Preps System.  The purified 788bp amplimers were used directly for fluorescent Big Dye sequencing. (2299)

Notes: Total RNA was extracted from HEL-5J20 and used to generate a full length cDNA (576bp) of the calcium- and integrin-binding protein using the Access RT-PCR System. The subsequent product was purified with the Wizard^® PCR Preps System, digested with restriction enzymes and cloned into a GST-fusion expression vector. (0214)

Notes: PCR products were purified with the Wizard® PCR Preps DNA Purification System and directly sequenced with the SILVER SEQUENCE™ DNA Sequencing System. (1334)

Notes: Plasmids were purified with either the Wizard^® Plus Minipreps DNA Purification System or the Wizard^® Plus Midipreps DNA Purifications System. PCR products were purified with the Wizard^® PCR Preps System. (0751)

Notes: PCR products were purified by Wizard^® PCR Preps DNA Purification System and sequenced by use of the fmol^® DNA Cycle Sequencing Systems. (0247)

Notes: PCR was used to amplify the VHL coding and promoter region in six fragments from genomic DNA, and SSCP analysis was performed to detect intragenic mutations. Fragments producing an aberrant SSCP band were purified using the Wizard^® PCR Preps DNA Purification System and subsequentially sequenced. (0176)

Notes: Bisulphite-treated DNA was purified using the Wizard® DNA Clean-Up System prior to PCR. The amplified DNA was then cleaned using a Wizard® PCR Preps DNA Purification System prior to cloning and the cloned DNAs were sequenced using the fmol® DNA Cycle Sequencing System. (0001)

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from 3ml S. cerevisiae cultures grown 18-20 hours in YPD broth. The isolated DNA was used for PCR amplification of a 3.5kb segment. The amplimer was purified using the Wizard® PCR Preps System. (0356)

Notes: Wizard^® PCR Preps DNA Purification System was used to purify PCR products. 100 ng of the PCR products was sequenced using the fmol^® DNA Cycle Sequencing System. (0488)

Notes: The Transfectam® Reagent was used to transiently transfect NIH 3T3, COS-7 and HeLa cells. A lot of detail is provided for the transfection method. Wizard® Plus Miniprep DNA Purification System and Wizard® PCR Preps DNA Purification System were used for plasmid purification and PCR product purification from low melting point agarose, respectively. (0226)

Notes: Chromosomal E.coli DNA was prepared with the Wizard^® Genomic DNA Purification Kit and used for PCR. The resulting PCR fragments were purified with the Wizard^® PCR Preps DNA Purification System and used for fluorescent sequencing. (0203)

Notes: PCR products were purified by Wizard^® PCR Preps DNA Purification System and sequenced with the fmol^® DNA Cycle Sequencing System. (0576)

Notes: The Serine/Threonine Phosphatase Assay System was used to assess protein phosphatase 2A, 2B and 2C activity in nuclear extracts of Human umbilical vein cells (HUVEC) with or without lysophosphatidylcholine treatment. Gel shifts were also performed with nuclear extracts prepared from the HUVEC cells using the SP1 Consensus Oligonucleotide. Luciferase reporter studies were also performed in HUVEC cells using pGL3 Basic-derived vectors and the Luciferase Assay System. (1305)

Notes: The Altered Sites^® II in vitro Mutagenesis System was used to replicate mutations identified in patients with Glanzmann's Thrombasthenia. The cDNA was cloned into the pALTER^®-1 Vector and all mutation were confirmed by sequencing with the fmol^® DNA Cycle Sequencing System. (0660)

Notes: Taq DNA Polymerase was used for PCR. The PCR products were purified with the Wizard^® PCR Preps DNA Purification System and cloned with the aid of the pGEM^®-T Vector System. (0624)

Notes: Tryptic peptides, produced with Sequencing Grade Modified Trypsin, of the purified lipase were used to generate primers for RT-PCR. The largest amplimer was purified and used to screen a lambda gt11 library. The isolated 303 amino acid clone and site-specific mutants were put into the pCI-neo Mammalian Expression Vector and expressed in COS cells. The transiently expressed proteins were assayed for esterase and lipase activity. (0960)

Notes: The authors use the Reverse Transcription System, Wizard^® PCR Preps DNA Purification Systems, Wizard^® Plus Minipreps DNA Purification System and the pGEM^®-T Vector System in their studies. (0445)

Notes: The Altered Sites^® II in vitro Mutagenesis System was used to introduce four individual amino acid changes into the subtilisin protein. The Wizard^® PCR Preps DNA Purification System was used as well. (0102)

Notes: The system was used to isolate poly A+ RNA for INS-1 cell total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used in RT-PCR and Northern analysis. (1700)

Notes: DNA with unmethylated cytosines converted to uracil by bisulfite treatment was purified with the Wizard^® PCR Preps DNA Purification System. The DNA was used for methylation-specific PCR. (0149)

Notes: The Dual-Luciferase™ Reporter System was used to quantitate the presenilin promoter activity in Neuro2a neuroblastoma cells, mouse P19 embryonal carcinoma cells and NIH 3T3 cells. Studies were also performed in P19 cells treated with retinoic acid to acquire a neuron-like phenotype and P19 cells treated with dimethyl sulfoxide to acquire a muscle-like phenotype. The presenilin promoter functioned best in the Neuro2a and neuron-like P19 cells. 5´-RACE products from mouse brain RNA were purified with the Wizard^® PCR Preps System and cloned into the pGEM-T Vector . The cloned amplimers were sequenced and used as a template for amplification to produce truncation mutants to assess promoter activity. (1590)