Notes: In this paper, JM109(DE3) cells were transformed with pBP06, a plasmid containing bovine pepsinogen cDNA under control of the T7 RNA polymerase-inducible promoter.  It was found that the best IPTG induction conditions for the cells were 16 hours at 20°C.   (3184)

Notes: The 1kb MR-1 decaheme cytochrome MtrA gene from Shewanella oneidensis was expressed in JM109(DE3) cells. Transformed cells were grown at 37°C until they reached an OD600 of 1.0. They were then induced with IPTG overnight.  (3183)