Notes: The SV Total RNA Isolation System was used to isolate total RNA from various mouse cells lines: DA3 IL-3 dependent myeloid cells; 7TD1 IL-6-dependent B-cell hybridoma; FM3A mammary carcinoma; L5287Y lymphoma; WEHI-3 myeloma; LLC1 Lewis lung carcinoma; L929 fibroblast; J774A macrophage-like cells. Five micrograms of each RNA was used for Northern analysis. The Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL System) was used to determine if HL60 were apoptotic when treated with retionic acid to induce growth arrest. Less than 10% of the cells were positive in the TUNEL assay (data not shown). (0113)

Notes: The Apoptosis Detection System, Fluorescein was used to identify apoptotic cells in C6 glioma cultures in response to various reagents. TUNEL Update: The Apoptosis Detection System, Fluorescein has been renamed to DeadEnd™ Fluorimetric TUNEL System. (0504)

Notes: The Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL Assay) was used to assess apoptosis in mitogen-activated T cells treated with the ether phospholipid. The phospholipid did not induce apoptosis in resting T cells. (1376)

Notes: The Apoptosis Detection System, Fluorescein was used to identify apoptotic primary olfactory bulb ensheathing cells. Cells were purified by FACS with a specific cell surface molecule, then plated onto poly-L-lysine coverslips. Good detail is provided for cell processing for the TUNEL assay. Update: The Apoptosis Detection System, Fluorescein has been renamed as DeadEnd™ Fluorometric TUNEL System (Cat.# G3250). (0539)

Notes: The Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL Assay) was used to monitor the apoptosis of H4 human neuroglioma cells following serum withdrawal and treatment with staurosporine. Over 40% of the cells were positive for TUNEL staining. (1504)

Notes: The Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL System) was used to label fragmented genomic DNA in the SH-Sy5Y neuroblastomas that had undergone shear stress. This TUNEL assay was used in conjunction with an LDH assay to confirm the mode of cell death. The TUNEL labeling was analyzed by flow cytometry. (0232)

Notes: Trypanosomes were treated with xanthine and xanthine oxidase to create reactive oxygen species. The cells were affixed to poly-L-lysine coated slides, fixed in 3% paraformaldehyde, and permeablized with 0.1% Triton® X-100 for TUNEL positive cells. TUNEL staining was provided by the Apoptosis Detection System, Fluorescein. Update: The Apoptosis Detection System, Fluorescein has been renamed DeadEnd™ Fluorometric TUNEL System. (0475)

Notes: The Apoptosis Detection System, Fluorescein, was used in triple fluorescent labeling. Bovine aortic endothelial cells were treated with colchicine and analyzed for the presence of β-Galactosidase (marker for transfection), TUNEL and nuclei (Hoechst dye). Transfection with a dominant negative mutant JNK (K-R) prevented the appearance of TUNEL staining in the cells and also eliminated the DNA ladder characteristic of apoptosis. The name of the Apoptosis Detection System, Fluorescein, has been changed to DeadEnd™ Fluorometric TUNEL System. (1025)

Notes: The Apoptosis Detection System, Fluorescein, was used to examine apoptosis in the retinas of cyclin D1 deficient transgenic mice. The name of the Apoptosis Detection System, Fluorescein, has been changed to DeadEnd™ Fluorometric TUNEL System. (0757)

Notes: The Apoptosis Detection System, Fluorescein was used to detect apoptosis in HL60 and K562 cells treated with etopiside, herbimycin A and a etopiside/herbimycin A mixture. The etopiside/herbimycin A mixture induced the most dramatic level of apoptosis on K562 as judged by the TUNEL assay and a cell morphology assay. The etopiside treatment of HL60 cells produced the most significant amount of apoptosis in this cell type. The degree of apoptosis in HL60 cells was dramatically reduced in the presence of herbimycin A. Herbimycin A treatment coupled with irradiation produced dramatic apoptosis of K562 cells. The same tests were also performed with Tom 1 and KCL-22 cells. Update: The Apoptosis Detection System, Fluorescein has been renamed to DeadEnd™ Fluorimetric TUNEL System. (0478)

Notes: The Apoptosis Detection System, Fluorescein was used to monitor apoptosis in motor neurons deprived of BDNF. Motor neurons were derived from day 15 rat embryos. Contains a figure of a apoptotic neuron stained with the fluoresceinated dUTP and propidium iodide. (1179)

Notes: Promega's Apoptosis Detection System, Fluorescein was used to confirm that the 786-0 human sporadic renal carcinoma cells were apoptotic following serum withdrawal. Staining with the TUNEL assay was used as a confirmation of the visual identification of pycnotic nuclei. The data is not shown in the paper.  Update: The Apoptosis Detection System, Fluorescein has been renamed to DeadEnd™ Fluorometric TUNEL System (Cat.# G3250). (0553)

Notes: The Apoptosis Detection System, Fluorescein was used for TUNEL assays on erythroblast clones. Cells were also stained with propidium iodide. The labeled cells were analyzed by flow cytometry to determine the percentage of apoptotic cells and the cell cycle distribution. TGFα (discontinued) was used to maintain erythroid progenitors in culture. Update: The Apoptosis Detection System, Fluorescein has been renamed to DeadEnd™ Fluorimetric TUNEL System. (0526)

Notes: Mutant and wildtype arginine vasopressin (AVP) constructs were co-transfected with a CAT-expression vector containing a neo resistance gene. Stable transformants of Neuro2A cells were generated with the aid of the Transfectam^® Reagent and G418 sulfate. CAT activity in stably transfected clones was determined with the CAT Enzyme Assay System. The effect of the mutant AVP constructs on cell viability was determined with the CellTiter 96^® AQ_ueous Cell Proliferation Assay (MTS/PMS). Several constructs displayed marked decreases in cell viability. The cause was necrosis rather than apoptosis due to the absence of DNA laddering and a negative reaction in the Apoptosis Detection System, Fluoroscein (data not shown). The name of the Apoptosis Detection System, Fluorescein, has changed to DeadEnd™ Fluorometric TUNEL System. (1009)