Notes: The induction of CYP1A1 in HepaRG and hepatocyte cells by mono-methylindoles was evaluated using the P450-Glo™ CYP1A1 assay. (5207)

Notes: A novel in vivo CRISPER/Cas9-based transcriptional activation (CRISPERa) tool, flySAM, is presented for D. melanogaster. FlySAM is more effective, scalable, and simple allowing for improved overexpression genetic analysis. Activation of transcription was monitored with a luciferase reporter and the Steady-Glo™ Luciferase Assay System. A collection of transgenic TRiP-OE CRISPRa stocks from flySAM is available at https://fgr.hms.harvard.edu/fly-in-vivo-crispr-cas. (5175)

Notes: A minimal promoter from the gene encoding BiP was cloned into the pGL3-Basic Vector. The resulting construct was transfected into HEK293 cells and, 48 hours post transfection, cell lysates were prepared using Glo Lysis Buffer. The Bright-Glo™ Luciferase Assay System was then used to assess levels of luciferase expression.  As a transfection control, cells were co-transfected with the pSV-β-Galactosidase Control Vector.  (3229)

Notes: HeLa and Saos-2 were transiently transfected with luciferase reporter vectors made from the pGL3-Promoter vector containing various intron sequences from the human integrinα3 gene ITGA3. The first intron sequence was originally cloned by PCR cloning into the pGEM®-T Easy vector. Cell cultures were assessed for luciferase activity by making lysates with the Glo Lysis Buffer and assaying with the Steady-Glo® Assay kit. (3225)