Notes: In this study, Poly(A)+ RNA was isolated from B16 melanoma cell total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for cDNA library construction. The RNAgents^® Total RNA Isolation System was used to isolate total RNA from HEL and K562 human erythroleukemia cells. The isolated RNA was used for RT-PCR. RNasin^® Ribonuclease Inhibitor was used in a very innovative fashion. Peripheral mononuclear cells isolated from blood were directly lysed in a hypotonic buffer containing the inhibitor. The lysates from these cells were used directly for RT-PCR. (2026)

Notes: The inhibitor was used to protect RNA during RT-PCR. (2246)

Notes: NGF was used in this study of neurite outgrowth in PC12 cells under a variety of conditions. Protein Kinase C was used to in vitro phosphorylate a bacterially expressed protein and the phospho protein was assayed by gel shift. Many details of the phosphorylation are provided. (0304)

Notes: Poly(A)+ RNA was isolated from mouse brain total RNA and used for Northern analysis. The authors used Promega's PolyATtract^® mRNA Isolation System, AMV Reverse Transcriptase and RNasin^® Ribonuclease Inhibitor in this study. (2104)

Notes: The inhibitor was used to protect RNA during RT-PCR. 125I-NGF was produced and used to crosslink to cell surface proteins on PC12 cells. The NGF was also used to assay for neurite outgrowth of PC12 and two clonal variants. Cells were treated with NGF and nuclear extracts made and used to assay for AP1 binding activity. Tyrosine phosphorylation of PI kinase in response to NGF was assayed. Also, the concentration-dependent effect of NGF on the accumulation of 3H-inositol phosphate was examined in the PC12 cells. The bFGF was used to assay the induction of cell differentiation in the PC12 cell and a clonal variant. (1632)

Notes: The Altered Sites^® II in vitro Mutagenesis System was used to create seven different mutant ssoA genes. The mutations also incorporated new restriction sites for easy screening. (0891)

Notes: The inhibitor was used to protect transcripts during a run-on transcription assay. (1638)

Notes: T7 RNA Polymerase and SP6 RNA Polymerase were used to produce RNA probes labeled with digoxygenin-11-UTP. Transcripts were made in the presence of RNasin^® Ribonuclease Inhibitor in a standard transcription reaction. The ribonucleotides ATP, CTP and GTP were used at 1mM. Both UTP and digoxygenin-11-UTP were used at 0.5mM in the reaction. The RNA probe was used for in situ hybridization. (1563)

Notes: Promega's Taq DNA Polymerase, RNasin^® Ribonuclease Inhibitor, and fmol^® DNA cycle Sequencing System were used in this study. (1952)

Notes: The AMV Reverse Transcriptase, RNasin^® Ribonuclease Inhibitor and Taq DNA Polymerase were used for RT-PCR to isolate a clone of the human insulin upstream factor 1, a transcription factor. The protein was expressed in vitro with the Rabbit Reticulocyte Lysate and used for gel shift assays. Reporter studies were performed in MIN6 cells. (0762)

Notes: Jurkat cells, a human T lymphocyte line that can be induced to synthesize and secrete interleukin 2, contain a factor that binds interleukin 2 mRNA. The binding is sequence specific and occurs in the 3’-non-coding region, within 160nt of the end of the coding region, at or near a site on the mRNA that is rich in A and U residues. However, it appears not to be due to known AU binding factors. The factor is protease sensitive and binds non-covalently to interleukin 2 mRNA. It behaves like aprotein of molecular weight 50,000-60,000 after UV-induced cross-linking to the mRNA. Preparations of the binding factor also protect interleukin 2 mRNA against degradation by a recently described RNasin-resistant endoribonuclease activity in Jurkat cells. Protection occurs under the same conditions required to generate the gel-retarded complex. (1645)

Notes: These authors used Promega's AMV Reverse Transcriptase, RNasin^® Ribonuclease Inhibitor and Taq DNA Polymerase in this study. (2061)

Notes: Promega's Taq DNA Polymerase, fmol^® DNA Cycle Sequencing System and RNasin^® Ribonuclease Inhibitor were used in this study. (1954)

Notes: Promega's Taq DNA Polymerase, RNasin^® Ribonuclease Inhibitor and M-MLV Reverse Transcriptase were used in this study. (2003)

Notes: Promega's RQ1 RNase-Free DNase, M-MLV Reverse Transcriptase and RNasin^® Ribonuclease Inhibitor were used in this study. (2020)

Notes: The cytosolic, alkaline RNase in rat vaginal epithelial cells (VEC) from normal, immature rats was found to be present largely in free, active form unlike in many other mammalian tissues where it is known to be present in a latent form as a complex with RNase inhibitor (RNasin). Estradiol (E2) administration induced expression of RNasin activity in the VEC from such animals and caused virtually total inhibition of cytosolic RNase activity in these cells by 12 h after hormone injection. These changes may have metabolic implications in relation to other biochemical events stimulated by estradiol in rat VEC. (1647)

Notes: The inhibitor was used for protection of cells and colonies following RT-PCR. (1672)

Notes: Lipopolysaccharide (endotoxin), a component of gram-negative bacteria, causes marked alterations in eukaryotic gene expression and cellular physiology. We show that within one hour of injection of endotoxin into adult rats, there is an induction of ribonuclease activity in the lung. The degradation of RNA was prevented by treatment of the lung extract from endotoxin-injected rats with ribonuclease inhibitor (RNasin). We suggest that induction by endotoxin of ribonuclease activity is a novel mechanism by which cells could alter gene expression to meet an environmental challenge and caution that the presence of ribonuclease can hinder molecular biological analyses of tissue extracts from endotoxin-treated rats. (1644)

Notes: RNasin Ribonuclease Inhibitor was used in the mRNA and protein localization steps. (4183)

Notes: Protein synthesis was restored to angiogenin-injected oocytes by injecting RNasin® Ribonuclease Inhibitor plus total Xenopus or calf liver tRNAs. (1635)

Notes: Recombinant angiogenin injected into oocytes abolished protein synthesis, and this toxic effect was inhibited by RNasin^® Ribonuclease Inhibitor. (2248)