Notes: The authors of this study used real time-qPCR and PCR-DGGE (denaturing gradient gel electrophoresis) to analyze and compare the mixed bacterial systems from fecal samples of vegetarians and omnivores. Bacterial DNA was isolated from frozen fecal samples using the Maxwell® 16 Tissue DNA Purification Kit, and PCR-DGGE reactions were set up using GoTaq® Flexi DNA Polymerase and GoTaq® Flexi Colorless Reaction Buffer. The authors of this study were able to detect differences in microbiota that seemed to be related to diet. (4523)

Notes: The authors were interested in quantitating the presence as well as the prevalence of Lactobacillus gasseri K7 in humans that did not consume the probiotic bacteria. Fecal samples from 45 healthy adults were collected, frozen, diluted, centrifuged and digested with proteases. After sonication, DNA was extracted using the Maxwell® 16 Tissue DNA Purification kit on the Maxwell® 16 instrument. This same kit and instrument also were used to isolate bacterial DNA from 1ml bacterial cultures. The purified DNA was PCR amplified using primers for gassericin K7 A and K7 B (bacteriocin) genes and GoTaq® Flexi DNA Polymerase in Green GoTaq® Flexi Buffer for 30 cycles. PCR products were analyzed by 1.8 % agarose gel electrophoresis. (4522)

Notes: Human Genomic DNA used as the starting material in the NGS workflow.  GoTaq® Flexi Colorless Buffer and GoTaq® Flexi Polymerase were used in amplification of template in step added to the beginning of library preparation. (4531)