Notes: The authors quantified purified retinal gDNA samples using the QuantiFluor® dsDNA System prior to whole-exome capture, library preparation and sequencing on an Illumina HiSeq 2500. (4803)

Notes: The authors quantified TruSeq cDNA libraries with the QuantiFluor® dsDNA System for sequencing on the Illumina HiSeq 2000. (4792)

Notes: The authors purified DNA and amplified into a library using the Illumina 16S Metagenomic Sequencing Library Preparation protocol. Concentration was determined by the Quantus™ Fluorometer and libraries were normalized, pooled and sequenced on an Illumina MiSeq. (4783)

Notes: The authors quantified DNA libraries using the Quantus™ Fluorometer and QuantiFluor® dsDNA System. The DNA libraries were normalized, pooled and sequenced on an Illumina MiSeq. (4784)

Notes: The authors purified DNA from expressed prostate secretions (EPS) and quantified using the QuantiFluor® dsDNA System. The purified DNA was made into Illumina libraries, quantified again with the QuantiFluor® dsDNA System, normalized to 10nM and sequenced on an Illumina NextSeq 500. (4802)

Notes: The authors extracted genomic DNA from bacteria using the Wizard® Genomic DNA Purification Kit and quantified using the QuantiFluor® dsDNA System. The DNA was made into libraries and sequenced with an Illumina HiSeq 2000. (4805)

Notes: The authors quantified TruSeq cDNA libraries with the QuantiFluor® dsDNA System and then performed RNA-seq using the HiSeq 2000. (4797)

Notes: The authors purified microbial DNA samples which were quantified with a Quantus™ Fluorometer and QuantiFluor® Dyes prior to using the Nextera™ DNA Library Prep Kit and sequencing on a MiSeq Instrument.  (4785)

Notes: The authors purified viral DNA from water samples and quantified using a Quantus™ Fluorometer prior to Nextera® DNA library preparation and Illumina MiSeq sequencing. Bacterial 16S rRNA genes were amplified by PCR from the same water samples, purified and then quantified with a Quantus™ Fluorometer prior to amplicon sequencing with a Roche Sequencing 454 GS Junior System. (4787)

Notes: The authors quantified 16S rDNA amplicon libraries using the QuantiFluor® dsDNA System and pooled libraries together for sequencing on a Genome Sequencing Junior System (454/Roche). (4793)

Notes: The authors quantified cDNA libraries with the QuantiFluor® dsDNA System for sequencing on the Illumina HiSeq 2000. (4790)

Notes: The authors quantified purified DNA from rhizopsphere soil samples using the QuantiFluor® dsDNA System prior to library creation and sequencing on an Illumina MiSeq. (4804)

Notes: The authors quantified purified apple leaf DNA samples using the QuantiFluor® dsDNA System and the GloMax®-Multi+ Detection System prior to Illumina sequencing. (4798)

Notes: The authors quantified cell line DNA using the QuantiFluor® dsDNA System prior to targeted capture, library preparation and Illumina sequencing. (4800)

Notes: The authors quantified TruSeq cDNA libraries with the QuantiFluor® dsDNA System for sequencing on the Illumina HiSeq 2000. (4791)

Notes: Th authors made purified bacterial DNA into amplicon libraries, quantified using the QuantiFluor® dsDNA System and sequenced on an Illumina MiSeq. (4801)

Notes: The authors extracted bacterial DNA from 3ml LB broth using the Wizard® Genomic 4 DNA Purification Kit and quantified with the QuantiFluor® dsDNA System prior to sequencing on an Illumina HiSeq 2000. (4796)

Notes: The authors quantified 16S rDNA amplicon libraries using the QuantiFluor® dsDNA System and pooled the libraries together for sequencing on an Illumina MiSeq (4794)

Notes: The authors immunoprecipitated chromatin, purified the DNA and quantified on a Quantus™ Fluorometer with QuantiFluor® dsDNA System. The DNA was made into libraries and sequenced on an Illumina HiSeq2000. (4788)

Notes: The authors purified DNA from oral mucosa samples. The DNA was bisulfate treated, made into libraries and quantified with a Quantus™ Fluorometer. The DNA libraries were normalized, pooled and sequenced on a Roche 454 Sequencing GS Junior System. (4789)

Notes: The authors quantified amplicon libraries using the QuantiFluor® dsDNA System and the GloMax®-Multi Detection System prior to pooling and sequencing on a 454-GS-FLX Titanium System. (4795)

Notes: The authors of this study set out to compare five automated systems for purification of DNA from FFPE tissue samples and five DNA quantification methods to determine the systems and methods most favorable to successful downstream massively parallel sequencing (next generation sequencing) analysis. Tissues were processed using commercially available DNA purification kits available for each platform. The authors concluded that the DNA extracted using the Maxwell® 16 systems and platform was of the highest quality in this study and gave the best results in downstream applications. (4512)

Notes: The authors of this study piloted use of uncomplicated analysis of the full chloroplast genome to study factors that affect the distribution of plant species. They looked at rainforest tree species in two distinct biogeographical areas in New South Wales, Australia. They extracted total genomic DNA from leaf samples, and quantified the DNA with the QuantiFluor™ dsDNA System on the SpectraMax Gemimi XPS detector before next generation sequencing. (4506)

Notes: This study investigated the effect of nitrate on nitrogenase activity in root nodules of Medicago truncatula . The authors monitored the decline in nitrogenase activity after nitrate exposure, then extracted and sequenced RNA from nodules at two time points: 1) When adequate nitrate to meet the plants needs was taken up; and 2) When nodule nitrogenase activity showed significant decline. Total RNA purified from nodules at these time points was used to construct cDNA libraries. The cDNA was quantified using the Quantifluor® dsDNA System prior to sequencing using the Illumina HiSeq 2000 Instrument. (4500)

Notes:

In this paper, the Quantifluor® dsDNA dye was used to quantify cDNA during construction of an RNA sequencing library and prior to sequencing on an Illumina HiSeq 2500 instrument. To understand how the gene activation landscape changes as abundance of the regulatory protein CodY decreases, the authors performed genome-wide transcript profiling of wild type B. subtilis strains and a series of mutants that had varying levels of CodY expression. Comparison of the activation profiles provided insights into how the genes regulated by CodY are controlled depending on the level of activation of CodY by its ligands.