Notes: HEK 293T cells were transfected with a reporter plasmid (assembled in the pGL3 Basic Vector) with a consensus BCL6 binding site and a BCL6 expression plasmid using the ViaFect™ Transfection Reagent (about 2µg of DNA per well in 6-well plates at 50-60% confluency). Promoter activation was monitored with a luciferase assay control with a β-galactosidase control. Western blotting of transfected cells used the Anti-Rabbit IgG (Fc), Alkaline Phosphatase Conjugate and the Western Blue® Stabilized Substrate for Alkaline Phosphatase. (4657)

Notes: The ability of the environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to upregulate cytochrome P450 CYP1A1 levels in mouse liver was examined. Total mouse liver RNA was reverse transcribed using the Reverse Transcription System, and the resulting CYP1A1 cDNA was quantitated using real-time PCR. CYP1A1 protein levels were quantitated by Western blot using an anti-CYP1A1 antibody, the Anti-Rabbit IgG (Fc), Alkaline Phosphatase Conjugate secondary antibody and the Western Blue^® Stabilized Substrate for Alkaline Phosphatase.
(3443)

Notes: Extracts were made from Rat-1 fibroblasts with or without IL-6 treatment. Lysates were prepared with Dignam C buffer minus the phosphatase inhibitors. The extracts were run over a G-25 spin column to remove free phosphate and were then assayed using the Serine/Threonine Phosphatase Assay System. Western Blue^® Stabilized Substrate for Alkaline Phosphatase was used for developing Western blots. (0350)

Notes: Immunoblots, on PVDF, were developed with the Anti-Rabbit IgG Alkaline Phosphatase Conjugate and the Western Blue^® Stabilized Substrate for Alkaline Phosphatase. (1200)

Notes: The Western Blue® Stabilized Substrate for Alkaline Phosphatase was used to develop immunoblots on nitrocellulose membranes. (1312)