Verrijzer, C.P., Yokomori, K., Chen, J-.L. and Tjian, R.
Notes: Specific protein-protein interactions were mapped in vitro using a variety of techniques, including co-immunoprecipitation. dTAFII150DeltaN, dTAFII150DeltaC, pTAF150DeltaN and pTAF150DeltaC were expressed in the TNT® Coupled Reticulocyte Lysate System in the presence of 35S label. Protein A-Sepharose® beads were bound to antibodies to dTAFII250 or to the HA epitope and incubated with HA-dTBP, HA-dTAFII110 and dTAFII250DeltaN fusion proteins expressed in baculovirus. The 35S-labeled proteins were incubated with the antibody-baculovirus-expressed protein complexes and then analyzed by SDS-PAGE. dTAFII150 was shown to bind efficiently to immobilized HA-tagged dTBP and dTAFII250DeltaN, but did not interact selectively with dTAFII110 in vitro. (1775)