Notes: The authors purified ctDNA from 1ml plasma using the Maxwell® RSC ccfDNA Plasma Kit with the Maxwell® RSC Instrument. Cell line mismatch repair deficiency was confirmed by the MSI Analysis System, Version 1.2. Cell line authentication was performed using the Cell ID™ and GenePrint® 10 Systems. DNA from cultured and treated cells were purified with the Wizard® SV and SV 96 Genomic DNA Purification Systems. Cetuximab-treated cells were measured for viability with the CellTiter-Glo® Luminescent Cell Viability Assay. (4777)

Notes: The effect of overexpression of MAP2K1 (D67N) was assessed in the Calu-1 human cells through transfection with ViaFect™ Transfection Reagent (details not provided). The effect of 72 hour cisplatin exposure on 13 different human lung cell lines was assessed with the CellTiter 96® AQueous One Solution Assay. All cells were authenticated through STR analysis using the GenePrint® 10 System. (4682)

Notes: Numerous cell lines were cultured for 72 hours in medium with glutamine, without glutamine or without glucose.  In all cell lines, glucose withdrawal was cytotoxic during the incubation.  The cells were highly tolerant of glutamine withdrawal with minimal cytotoxicity.  Cytotoxicity was measured with the CellTox™ Green Cytotoxicity Assay added at plating to cells in 12 well plates.  The fluorescence was measured every hour while incubated in an Essen BioScience IncuCyte FLR instrument. All cell lines used in the study were authenticated through STR analysis with the GenePrint® 10 System.  (4646)