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Molecular Cloning is the process of producing recombinant DNA and transforming into host organisms to replicate and make more copies. Every cloning project is unique. Read More

Restriction Enzymes

Name	Description
4-CORE Buffer Pack	Open/Close	Add
Majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers
The 4-CORE Buffer Pack contains convenient aliquots of Promega Restriction Enzyme 10X Buffers A, B, C and D. The majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers. Visit Product Page »
AgeI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix
A/CCGG T T GGCC\A Visit Product Page »
BamHI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning-qualified, providing a higher level of quality control for enzymes used in cloning applications
G/GATC C C CTAG\G Visit Product Page »
BclI	Open/Close	Add
Genome qualified to ensure optimal performance in genomic analysis applications
T/GATC A A CTAG\T Visit Product Page »
BglI	Open/Close	Add
Genome qualified to ensure optimal performance in genomic analysis applications
GCCN NNN/NGGC CGGN\NNN NCCG Visit Product Page »
DpnI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less
G(me)A/TC CT\(me)AG Visit Product Page »
EcoRI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Available at high concentration, containing 25,000 units of EcoRI at a concentration of 40–80u/μl
G/AATT C C TTAA\G Visit Product Page »
EcoRV	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
GAT/ATC CTA\TAG Visit Product Page »
HindIII	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
A/AGCT T T TCGA\A Visit Product Page »
HinfI	Open/Close	Add
Restriction endonuclease from Haemophilus influenzae Rf
G/ANT C C TNA\G Visit Product Page »
KpnI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
G GTAC/C C\CATG G Visit Product Page »
MluI	Open/Close	Add
Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
A/CGCG T T GCGC\A Visit Product Page »
MULTI-CORE Buffer Pack	Open/Close	Add
Enables digestion of DNA with more than one enzyme in a single reaction Only modest adjustments in the amount of enzyme used will ensure complete multiple digestions
The MULTI-CORE Buffer Pack contains convenient aliquots of the Promega universal restriction enzyme 10X buffer. The MULTI-CORE Buffer is formulated to provide simple buffering conditions for performing multiple digestions. Many Promega restriction enzymes have between 50% and 100% activity in reactions using MULTI-CORE Buffer. Visit Product Page »
NcoI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
C/CATG G G GTAC\C Visit Product Page »
NdeI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix
CA/TA TG GT AT\AC Visit Product Page »
NheI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Genome qualified to ensure optimal performance in genomic analysis applications
G/CTAG C C GATC\G Visit Product Page »
NotI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
GC/GGCC GC CG CCGG\CG Visit Product Page »
PstI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, provides a higher level of quality control for enzymes used in cloning applications
C TGCA/G G\ACGT C Visit Product Page »
PvuI	Open/Close	Add
Active and capable of digestion directly in GoTaq® Green Master Mix
CG AT/CG GC\TA GC Visit Product Page »
RsaI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less
GT/AC CA\TG Visit Product Page »
SacI	Open/Close	Add
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
G AGCT/C C\TCGA G Visit Product Page »
SalI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
G/TCGA C C AGCT\G Visit Product Page »
SgfI	Open/Close	Add
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
GCG AT/CGC CGC\TA GCG Visit Product Page »
SmaI	Open/Close	Add
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
CCC/GGG GGG\CCC Visit Product Page »
SpeI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
A/CTAG T T GATC\A Visit Product Page »
TaqI	Open/Close	Add
Restriction endonuclease from Thermus aquaticus YTI
T/CG A A GC\T Visit Product Page »
XbaI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
T/CTAG A A GATC\T Visit Product Page »
XhoI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
C/TCGA G G AGCT\C Visit Product Page »