Cell Line Identity – Prevent Cell Misidentification In Your Daily Lab Routine
In this webinar, you will learn about:
- Cell misidentification - What's that?
- Causes and consequences of working with the wrong cell line
- How to choose a cell authentication method
- Background of STR amplification and capillary electrophoresis (CE) for successful cell authentication and how to apply these methods correctly
Summary
In 1951 HeLa cells entered cell culture labs worldwide as the first human cell line. 70 years later, we know that at least 129 cell lines are contaminated with HeLa cells, with dramatic implications on the validity of past cell culture-related research. The fact that at least 509 cell lines are misidentified (ICLAC 2020) reveals a significant need for reliable cell authentication that is increasingly required by scientific journals and auditing authorities. Join this webinar to learn more about preventing cell misidentification by incorporating Good Cell Culture Practice (GCCP) into your daily routine. Short Tandem Repeat (STR) genotyping analysis is a widely applied strategy for identifying human cell lines and is the method of choice as recommended by the ATCC Standards Development Organization. During the webinar, we will introduce the state-of-the-art solutions for STR amplification and capillary electrophoresis (CE) that enable cell in-house cell authentication.
Register for this joint Promega and Eppendorf webinar to learn more about preventing cell misidentification by incorporating Good Cell Culture Practice into your daily routine.
Speakers
Jessica Wagener, PhD
Application Specialist Workflow Solutions
Eppendorf SE
Dr. Jessica Wagener is an Application Specialist focusing on Cell Handling at Eppendorf Headquarter in Hamburg, Germany. Entering a cell culture laboratory as a graduate student for the first time in 2006, she came to Eppendorf with 5+ years of experience in cell culture. After studying Biology at the Universities of Marburg and Düsseldorf, she investigated apoptosis mechanisms in different cell lines for her diploma thesis. Following one year as a Research Associate working with primary cells she started to work towards her PhD with a focus on reproductive biology at the Medical School Essen. She joined Eppendorf in April 2012 shortly after completing her PhD in Biology.
Gabriela Saldanha
Strategic Commercialization Manager Genomic Solutions
Promega
Gabriela Saldanha is a Strategic Commercialization Manager with the Genomic Solutions team. Gabriela's career spans over 20 years in the laboratory and in product development, strategy and implementation related to nucleic acid amplification, expression analysis and genotyping, more recently leading the Spectrum Compact CE System launch and commercialization.