Introduction to Nucleic Acid Purification: Purification Basics and Their Application to Different Sample Types
See how protocols can be optimized for specific sample types including:
- Plasmid preps
- Purification of nucleic acid fragments
- Purification of nucleic acid from blood
Summary
Purification of intact, amplifiable nucleic acid is a key step in many laboratory processes. Samples all progress though a common set of manipulations during purification: lysis, clearing, binding, washing and elution. Optimization of extraction methodologies is key for success, especially with challenging sample types and demanding downstream applications. Learn the basic steps and considerations for successful purification and see how protocols can be optimized for specific sample types including plasmid preps, purification of nucleic acid fragments and purification of nucleic acid from blood.
This webinar will be useful for any researcher interested in learning more about nucleic acid purification, the chemistries utilized in the steps of nucleic acid purification and approaches that can be taken when considering purification of nucleic acid from a novel source.
Speaker
Technical Services Scientist
Promega Corporation
Paraj is a Technical Services Scientist at Promega Corporation based in Madison, WI. He received a B.S. in Microbiology and Public Health through Lyman Briggs College at Michigan State University, and a M.S. in Genetics from the University of Wisconsin-Madison. As a graduate student, Paraj researched tumor suppressor genes in mice, and Mutation accumulation in Brassica. Paraj has been with Promega for over 17 years, mostly working in the research, design, and development of DNA purification technologies.