RealTime-Glo™ MT Cell Viability Assay

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G9712_RealTime-Glo--MT-Cell-Viability--10X100_3
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Monitor Cell Viability in Real Time

  • Save time, cell culture and reagent costs
  • Rapidly detect changes in cell viability

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Catalog number selected: G9711

$ 184.00
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This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

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RealTime-Glo™ MT Cell Viability Assay
100 reactions
$ 184.00
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Why Use the RealTime-Glo™ MT Cell Viability Assay?

With the RealTime-Glo™ MT Cell Viability Assay, you can monitor cell viability continually in the same sample well up to 72 hours to obtain more information about the mode of action of a treatment with regard to time and dose dependence. The assay reagent can be added to cells during plating, during treatment or at the end of treatment. No cell washing, media removal or further additions are required.

The nonlytic nature and rapid response of the assay make it possible to monitor cell viability over time in the same well, enabling easy multiplexing with other cell-based assays or downstream applications. The assay measures the reducing potential of viable cells and is ATP-independent, providing an orthogonal method for viability or cytotoxicity determination.

Learn how the RealTime-Glo™ MT Cell Viability Assay can be used to assess drug toxicity in organoids.

View Article
realtime-glo viability assay mechanism

The RealTime-Glo™ Assay Measures Reducing Potential, A Well-Established Indicator of Cell Viability

Assay Principle

In the RealTime Glo™ MT Cell Viability Assay, a non-lytic NanoLuc® Luciferase reaction occurs in the culture medium. NanoLuc® Luciferase and MT Cell Viability Substrate are added to cell culture media. The MT Cell Viability Substrate then diffuses into cells where it is reduced to form a NanoLuc® Substrate, which exits the cell and is used rapidly by NanoLuc® Luciferase in the media. Only metabolically active cells can reduce the substrate, and light production is proportional to the number of live cells in culture. Dead cells cannot reduce the substrate.

realtime-glo detects live cells 12420MB-W

Save Time, Cell Culture and Reagent Costs

One RealTime-Glo™ Assay Plate Yields the Same Data as Many Endpoint Assay Plates

Five hundred A549 cells were plated in medium containing the RealTime-Glo™ MT Reagents in 384-well plates and various concentrations of bortezomib. The luminescence signal was determined from each well every hour for 72 hours in a Tecan M200 plate reader with gas control module (37°C/5% CO2).

Real time live cell data collection 12466MF-W-a
bortezomib-live-dead-cell data-12466MF-W-b

Data from specific time points plotted to calculate EC50 at these time points.

Rapidly Detect Changes in Cell Viability

The RealTime-Glo™ MT Cell Viability Assay generates a luminescent signal that is dependent upon continuous reduction of the MT cell viability substrate by viable cells and rapid turnover by NanoLuc® Luciferase. Conventional assays based on reducing potential (alamarBlue, MTT) are accumulation-based and do not allow real-time detection of cell death in the same well. In this experiment, A549 cells were treated with 1% Triton® X-100. Viability assay reagents were applied and incubated for the indicated times post-treatment.

12613MA

Add to Cells at Plating, Dosing or Endpoint

Flexible Assay Setup

Perform real-time cell viability monitoring or endpoint detection. You decide how to set up your assay. Add the RealTime-Glo™ Reagent to cells at any time point when cell viability readings are desired. The assay is compatible with high-throughput screening in 96-, 384- and 1536-well plates.

Realtime kinetic or endpoint viability methods 12474MB-W

Multiplex with Other Assays for More Information Per Sample

The nonlytic, live-cell RealTime-Glo™ Assay is compatible with downstream applications and allows multiplexing with other assays, conserving precious samples and providing more informative data per sample.

Realtime-Glo viability data-etoposide-12573MD-W-a
Realtime-cytotoxicity-etoposide-data-12573MD-W-b

Protocols

Complete Protocol

Specifications

You are viewing: G9711 Change Configuration

What's in the box?

Item Part # Size

NanoLuc® Enzyme, 1,000X

 E499A 1 × 10μl

MT Cell Viability Substrate, 1,000X

 G971A 1 × 10μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G9712 Change Configuration

What's in the box?

Item Part # Size

NanoLuc® Enzyme, 1,000X

 E499A 10 × 10μl

MT Cell Viability Substrate, 1,000X

 G971A 10 × 10μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G9713 Change Configuration

What's in the box?

Item Part # Size

NanoLuc® Enzyme, 1,000X

 E499B 1 × 100μl

MT Cell Viability Substrate, 1,000X

 G971B 1 × 100μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

Resources

Articles

Citations

Posters

Other Resources

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