Notes: These authors used the RealTime-Glo™ MT Assay to evaluate the effect of the rare-earth metals lanthanum and cerium on viability in HepG2 and HT-29 cells. (4942)

Notes: These authors describe the design, assembly and toxicant response of multi-cellular 3D hepatic organotypic culture models. In high-throughput screening, changes in cell viability were determined over 24 hours using the RealTime-Glo™ MT Cell Viability Assay. Mechanism of cell death was determined with the ApoTox-Glo™ Triplex Assay, and GSH concentration was measured using the GSH-Glo™ Glutathione Assay.  (5011)

Notes: The human bio-artificial muscle (BAM) model was used to monitor cellular response to intramuscular drug injection. The RealTime-Glo™ MT Cell Viability Assay was used to observe pro-NanoLuc® substrate dispersal within the tissue after compound injection. Luminescence was monitored on the GloMax®-Multi Microplate Multimode Reader. (5181)

Notes: Researchers evaluated the use of magnetic nanoparticles and electromagnetic devices as a drug delivery approach with a possibility to treat cardiovascular disease. The biological effect on the cardiac system was evaluated using cardiomyocytes and measuring changes in cell viability, cytotoxicity, apoptosis and Reactive Oxygen Species (ROS) generation. (5029)

Notes: In this study, cell proliferation was measured every 12 hours using the RealTime-Glo™ MT cell viability assay. (4868)

Notes: In this study, the Real-Time-Glo™ MT Cell Viability Assay was used to examine cell survival under hypoxic conditions.  (4867)

Notes: Chemical-induced cytotoxicity was investigated in HEK293 and HepG2 cells using two real-time assay technologies: the Promega RealTime-Glo™ MT Cell Viability Assay and the Promega CellTox™ Green Cytotoxicity Assay.  (4870)

Notes: Cell viability was determined using the RealTime-Glo™ MT Cell Viability Assay Kit. (4871)

Notes: In this paper, the RealTime-Glo™ and CellTiter-Glo® cell viability assays were used to measure viability of various breast cancer cell lines cultured in the OrganoPlate® microfluidic chip system from Mimetas. The toxicity of different chemicals on three different breast cancer cell types grown in 2D and 3D culture was evaluated. The RealTime-Glo™ assay was useful for measuring viability on day 0 prior to drug exposure and after 3 days of treatment. The CellTiter-Glo® Assay also was used, limited to the endpoint approach. (4934)

Notes: An ethanol extract of the Asian Elm tree was examined. To ensure that the extract did not cause nonspecific cell death, HUVEC cells were cultured with various concentrations of the extract and monitored for changes in viability over a 24-hour period. The RealTime-Glo™ MT Cell Viability Assay reagents were added at dosing and viability monitored at desired time points; only the 24-hour time point is shown. (4700)

Notes: The RealTime-Glo™ MT Cell Viability Assay was used to assess the growth of L929 murine fibroblast onto a synthetic scaffold. The flat 4 ×4 mm² scaffolds were seeded with 5 ×10³ cells and monitored for proliferation over 48 hours with periodic readings. Growth on standard tissue culture-treated plates was used as a control. (4698)

Notes: Human adipose-derived stem cells were cultured in the presence or absence of the Integra® collagen-based scaffold and monitored for proliferation over a 144-hour period. Cells were seeded with 1,000 cells using the RealTime-Glo™ MT Cell Viability Assay reagents and luminescence monitored every 24 hours. (4702)

Notes: The authors used the CellTiter-Glo® Cell Viability Assay and CellTox™ Green Cytotoxicity Assay to monitor the reproducibility of their acoustic dispensing. The authors report that adding CellTox™ Green Cytotoxicity Assay at plating "gives more solid signals than adding at the end of the assay” and speculate that the dye stabilizes the DNA of dead cells. The CellTiter-Glo® Assay data supports that the dye is nontoxic in the 72-hour incubation. The authors routinely predispense 12.5nl of CellTox™ Green with preplated compounds into 1536-well assay plates using an Echo 550 device and store plates under nitrogen gas in storage pods to produce assay-ready plates awaiting cell additions. The authors also report preliminary results of using the nonlytic RealTime-Glo® Viability Assay and find highly correlated results with the lytic CellTiter-Glo® Assay. The authors relate that use of the nonlytic RealTime-Glo® and CellTox™ Green Assays open the possibility of doing further work with the same cells. (4703)

Notes: This study investigated the reason why cancers become resistant to anti-cancer treatments over time. Non-small-cell lung cancers were cultured for weeks in the presence of EGF receptor inhibitors and resistant clones obtained based on a specific mutation in the EGF receptor. Clones that became resistant within 6 weeks were found to result from cells already containing the EGFR mutation. Clones taking about 24 weeks to become resistant evolved from the EGFR mutation. RealTime-Glo™ MT Cell Viability Assay was employed in several studies over a 10-week period to monitor proliferation (data presented in Supplementary Figures). Each week prior to media change, RealTime-Glo™ Assay reagents were added, incubated for 1 hour and luminescence measured. After 72-hour incubations, short-term viability was measured with the CellTiter-Glo® Luminescent Cell Viability Assay. (4699)

Notes: The authors of this paper describe the application of a non-radioactive, bioluminescent assay to measure glucose uptake. The assay, now available as the Glucose Uptake-Glo™ Assay, showed similar sensitivity and produced comparable results to isotopic methods and is amenable to HTS. Additionally the authors performed the glucose-uptake assay in multiplex with the RealTime-Glo® MT Cell Viability Assay to obtain more information on the health of the cells. Luminescent assay results were measured using the GloMax® Discover Detection System. (4762)

Notes: In this experiment, cells were exposed to various concentrations of methylmercury, endosulfan, or 2,5-hexanedione for 24 hours. Then, the RealTime-Glo™ Assay was used to assess cell viability.  (4866)

Notes: RealTime-Glo™ MT Cell Viability Assay determined 3 days’ cell proliferation of the 3D printed constructs.  (4873)

Notes: The authors were investigating new methods of biofabrication of 3D multicellular constructs.  Cells were incorporated into a macroporous scaffold, encapsulated with alginate and printed onto a cold surface with agarose.  The viability immediately after printing was monitored hourly over 72 hours with the RealTime-Glo™ MT Cell Viability Assay. Total cell viability in the 3D constructs was monitored at day 2, 7 and 14 with the CellTiter-Glo® 3D Cell Viability Assay. (4819)

Notes: Cell viability was assessed using the Real-Time-Glo™ MT Cell Viability Assay. (4865)

Notes: RealTime-Glo™ MT Cell Viability Assay was used to demonstrate the anti-proliferative properties of aflibercept (a chimeric glycoprotein consisting of the ligand-binding elements of VEGFR1 and VEGFR2 fused to the human IgG1 Fc) on galactin-1- and VEGF-A-stimulated human retinal microvascular endothelial cells (HRMEC). The authors do not indicate the length of the incubation or whether an end-point or real-time monitoring method was used. (4701)

Notes: The authors describe a homogeneous, nonlytic, bioluminescent assay that measures cell viability in real time. They monitored cell health for 72 hours from the same test samples, distinguished differential cell growth, and investigated drug mechanism of action by analyzing time- and dose-dependent drug effects. The real-time measurements enabled them to detect cell death immediately (>75% signal decrease within 15 minutes of digitonin addition), analyze drug potency versus efficacy, and identify cytostatic versus toxic drug effects.They then screened an oncology compound library (Z′ = 0.7) and identified compounds with varying activity at different time points. (4590)

Notes: The effect of tryptophan on skin hTERT immortalized normal keratinocytes was assessed at 24 and 48 hours with the RealTime-Glo™ Cell Viability Assay.  After the read at 48 hours, CellTox™ Green Cytotoxicity Assay was added to each well to measure dead cells per well using the end-point protocol. Tryptophan, which was determined toxic to Pseudomonas biofilms, was not cytotoxic to the human keratinocytes. (4644)