LDH-Glo™ Cytotoxicity Assay

J2380_LDH-Glo Cytotoxicity Assay-10ml_3
customize-this-small
automation-support-promo

Simple, More Sensitive LDH Assay Method to Determine Cytotoxicity

  • Detects LDH release from small numbers of cells, including 3D cell models
  • Monitors cytotoxicity from the same sample over time
  • Provides more data per well through multiplexing with other cell-based assays

Size

Catalog number selected: J2380

$ 304.00
Your price:
Add to Cart
This product is discontinued
Request a Sample
Not available in all countries Add to Helix
This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
LDH-Glo™ Cytotoxicity Assay
10ml
$ 304.00
Your price: Log in
Change Configuration

Monitor Cytotoxicity from Small Numbers of Cells

The LDH-Glo™ Cytotoxicity Assay is a bioluminescent plate-based assay for quantifying lactate dehydrogenase (LDH) release into the culture medium upon plasma membrane damage. The bioluminescent detection is more sensitive than colorimetric or fluorescent methods, allowing accurate detection of LDH from a small number of cells, including primary cells and 3D cell cultures.

This LDH assay involves removing only a small amount of cell media (2–5µl) from each treated well, allowing you to get more data by sampling the same well over time, and by using the remaining media and cells for other cell-based assays.

How the Assay Works

LDH released from damaged cells catalyzes the oxidation of lactate with concomitant reduction of NAD+ to NADH. Reductase uses NADH and reductase substrate to generate luciferin, which is converted to a bioluminescent signal by Ultra-Glo™ rLuciferase. The luminescent signal generated is proportional to the amount of LDH present.
Lactate dehydrogenase detection with LDH-Glo cytotoxicity assay 15079ma-w

"The Promega LDH-Glo™ Assay is a reliable and easy-to-follow kit to use when measuring cell death. We use this in our lab and, compared to others, it really helps us get reliable data. Overall, we would highly recommend this kit."


LDH-Glo Customer

Monitor Cytotoxicity in 3D Cell Cultures

The LDH-Glo™ Cytotoxicity Assay is well-suited for measuring LDH released from small numbers of membrane-damaged cells. Here is an example of measuring drug-induced toxicity in HCT116 spheroids. The time-dependent toxicity measurements were performed by repeatedly sampling media from the same wells.
Measuring drug-induced toxicity in HCT116 spheroids using the bioluminescent ldh assay 15086mb-wb
HCT116 spheroids were formed using 2,500 cells/well in Corning 384-well Ultra-Low Attachment plates and treated with Doxorubicin. 2.5µl samples were collected from the same wells at the indicated time points and frozen in LDH Storage Buffer at a 1:10 dilution. Samples were thawed and further diluted 2.5-fold in LDH Storage Buffer before measuring LDH levels.
See more data: Learn how this assay is used with different model systems, including SKBR3 cells to assess an antibody drug conjugate and in 3D human liver microtissues to monitor cytotoxicity over time, in the poster: New Sensitive Luminescent LDH-release Assay that Enables “Real-Time” Sampling from Individual 3D Microtissues, presented at SLAS Europe 2018.
Download Poster

Detect Target Cell Cytotoxicity in ADCC Assays

The LDH-Glo™ Cytotoxicity assay can be used to assess cell death due to many mechanisms of action of biologics treatments, including complement dependent cytotoxicity (CDC), antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-drug conjugate cytotoxicity. In this example, the LDH-Glo™ Assay was used to detect ADCC killing of target cells using the biologic rituximab.
 
Detection of target cell cytotoxicity in ADCC assays using a bioluminescent ldh assay method 15087mb-wa
Cells were plated at a 20:1 Effector (PBMC):Target (Daudi) ratio and treated with varying concentrations of rituximab (RTX) in triplicate for 4 or 6 hours. Medium samples (2.5µl) from the same wells were removed and diluted into LDH Storage Buffer (47.5µl) and frozen. 25µl of thawed samples were added to an equal volume of LDH Detection Reagent and luminescence was recorded after 60 minutes incubation at room temperature. Concentration of rituximab versus RLU was plotted and data was fit to 4PL curve to calculate EC50. 
Detection of target cell cytotoxicity in ADCC assays using a bioluminescent ldh assay method 15087mb-wb
RLU values from control wells (performed on the same plate as the rituximab treatment) indicate that there is minimal luminescent signal from target or effector cells alone, and that addition of rituximab is required for cell killing. ADCC = Effector cells +Target cells + RTX. RLU = Relative Luminescence Units.

Protocols

Complete Protocol

Specifications

You are viewing: J2380 Change Configuration

What's in the box?

Item Part # Size

Reductase Substrate

 G885A 1 × 55μl

Lactate Dehydrogenase

 J195A 1 × 1 each

LDH Detection Enzyme Mix

 J238A 1 × 10ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Specifications

You are viewing: J2381 Change Configuration

What's in the box?

Item Part # Size

Reductase Substrate

 G885B 1 × 275μl

Lactate Dehydrogenase

 J195A 1 × 1 each

LDH Detection Enzyme Mix

 J238B 1 × 50ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Resources

Articles

Citations

Other Resources

Similar Products

CellTox™ Green Cytotoxicity Assay

Measures changes in membrane integrity. Kinetically monitors cytotoxicity up to 72 hours with multiplex capability.

G8741, G8742, G8743, G8731

CytoTox-Glo™ Cytotoxicity Assay

Highly sensitive luminescent cytotoxicity assay that measures the relative number of dead cells.

G9290, G9291, G9292

CytoTox-ONE™ Homogeneous Membrane Integrity Assay

A homogeneous, LDH-based fluorometric method for quantifying non-viable cells.

G7890, G7891, G7892

CytoTox-Fluor™ Cytotoxicity Assay

A single-reagent-addition, homogeneous, fluorescent assay that measures the relative number of dead cells in cell populations.

G9260, G9261, G9262

Frequently Used With

CellTiter-Glo® 3D Cell Viability Assay

A homogeneous method optimized to assess viability in 3D cell culture.

G9681, G9682, G9683

GloMax® Discover System

High-performance microplate reader for detecting luminescence, fluorescence and absorbance.

GM3000

RealTime-Glo™ MT Cell Viability Assay

A bioluminescent method to kinetically monitor viability in cell culture up to 72 hours.

G9711, G9712, G9713

Caspase-Glo® 3/7 Assay System

An easy-to-use, plate-based luminescent assay for detecting caspase-3/7 activity.

G8090, G8091, G8093, G8092, MG1010

Let's find the product that meets your needs.

Talk to a Scientist

Uwe

Uwe

Germany
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.