HaloTag® Mammalian Protein Purification System

G6790_HaloTag-Mammalian-Protein-Purification
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Recover Highly Pure Recombinant Protein from Mammalian Cell Cultures, Even at Low Expression Levels

  • HaloLink™ Resin covalently binds >7mg/ml of HaloTag® fusion protein (10X more capacity than FLAG®)
  • Easy to scale up or down to obtain milligram + quantities of protein
  • Choose from purification, purification + detection or sample purification + detection kits

Choose Mammalian Purification Kit

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Catalog number selected: G6790

$ 715.00
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HaloTag® Mammalian Protein Purification System
Protein Purification/1 each
$ 715.00
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Streamline Your Protein Purification Process

The HaloTag® Mammalian Protein Purification System (Cat.# G6790) is an optimized kit for purification of HaloTag® fusion proteins from mammalian cell culture lysates. HaloTag® fusion proteins form a highly specific and covalent bond with the HaloLink™ Resin. The covalent binding coupled with the low nonspecific binding of the HaloLink™ Resin provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag® Mammalian Protein Detection and Purification System (Cat.# G6795 or G6799) also includes HaloTag® TMRDirect™ Ligand. The simple-to-use fluorescent detection of the HaloTag® fusion facilitates rapid optimization of expression and purification conditions.

Streamlined purification process leads to higher purity and recovery of recombinant proteins from cultured cells.

Streamlined purification process leads to higher purity and recovery of recombinant proteins from cultured cells.

Protocols

Complete Protocol

Quick Protocols

Specifications

You are viewing: G6790 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

HaloLink™ Resin

 G1913 1 × 2.5ml

Protease Inhibitor Cocktail, 50X

 G652A 2 × 1ml resuspended View Product

HaloTEV Protease

 G660A 1 × 200μl 5u/μl View Product

Spin Column

 V995A 1 × 25 each

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

 

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

Specifications

You are viewing: G6795 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

HaloLink™ Resin

 G1913 1 × 2.5ml

HaloTag® TMRDirect™ Ligand

 G299A 1 × 30μl View Product

Protease Inhibitor Cocktail, 50X

 G652A 2 × 1ml resuspended View Product

HaloTEV Protease

 G660A 1 × 200μl 5u/μl View Product

Spin Column

 V995A 1 × 25 each

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

 

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

Resources

Citations

Similar Products

HaloTag® Protein Purification System

Enhanced expression and solubility of recombinant proteins with HaloTag® Ligands.

G6280, G6270

HaloTag® Mammalian Pull-Down Systems

These systems enable rapid capture of simple or complex protein pairs directly from lysates and allow you to go from pull-downs to imaging studies using same construct.

G6509, G6500, G6504, G6591

HaloLink™ Resin

HaloTag® ligand bound to Sepharose beads that specifically and rapidly binds HaloTag® fusion proteins.

G1912, G1913, G1914, G1915

Frequently Used With

HaloTEV Protease

Fusion of HaloTag® protein and TEV protease enables immobilization on HaloLink™ Resin and removal in a single-step purification.

G6601, G6602

HaloTag® Fluorescent Ligands

For protein labeling and detection. Each includes a chloroalkane group for HaloTag® protein attachment.

CS3495B06, CS3495B08, G8251, G8252, G2801, G2802, G8272, G8273, G8581, G8582, G1001, G1002, G8471, G8472, G2991, G3221, G8281, G8282, G8591, G8592

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