HIV HiBiT-PsVLP Bioassay
Measure Potency and Stability of Inhibitors that Bind and Block Viral Entry into Target Cells
- Safe: No virus generation; suited for BSL-1/2 environments
- Fast: Rapid, complementation-based readout
- Simple: “Add-mix-read” protocol—no wash steps
- Convenient: Reagents provided in Thaw-and-Use format
HIV HiBiT-PsVLP Bioassay: Complete Kits
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| CS3368A29 | Early Access HIV Env (JRFL) HiBiT-PsVLP, Complete Kit | View Specifications | 1 each | Please Enquire | |||
| CS3368A30 | Early Access HIV Env (JRFL) HiBiT-PsVLP, Complete Kit 5X | View Specifications | 1 each | Please Enquire |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
HIV HiBiT-PsVLP Bioassay: Standalone Vials
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| CS3368A24 | Early Access HIV Env (JRFL) HiBiT-PsVLP 1X | View Specifications | 1 each | Please Enquire | |||
| CS3368A25 | Early Access HIV Env (JRFL) HiBiT-PsVLP 5X | View Specifications | 1 each | Please Enquire | |||
| CS3368A27 | Early Access HIV HEK293T (LgBiT) Target Cells 1X | View Specifications | 1 each | Please Enquire | |||
| CS3368A28 | Early Access HIV HEK293T (LgBiT) Target Cells 5X | View Specifications | 1 each | Please Enquire | |||
| CS3368A32 | Early Access HIV HEK293T (LgBiT) Target Cells, Propagation Model | View Specifications | 1 each | Please Enquire |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
HIV HiBiT-PsVLP Bioassay: Measure Viral Neutralization With Ease
Neutralizing antibodies, whether generated through vaccination or produced as therapeutic monoclonal antibodies, are critical for stopping acute and chronic viral infections. Viruses like HIV are important targets for researchers developing vaccines, prophylactic monoclonal antibodies or small-molecule inhibitors, and they need to determine the neutralizing capacity of their therapeutic. However, current methods like PRNT or PNA require infection-competent virus, stringent biosafety measures and cannot be readily implemented in most laboratories.
The HIV HiBiT-PsVLP Bioassay is a simple, easily implemented, robust system that can be used to measure the viral neutralization activity of both antibody and small-molecule inhibitors that block viral entry into target cells. The assay consists of an HIV PsVLP and a target cell line that each contain one piece of a split luciferase technology to provide a low-background and convenient luminescent readout.
Demonstrate HIV Neutralization By Blocking HIV Env Binding to Surface Receptors
Principle of HIV HiBiT-PsVLP Bioassay. 1. HiBiT-tagged VLPs pseudotyped with HIV Env protein are added to the HIV (LgBiT) Target cells. 2. In the presence of inhibitors or neutralizing antibodies (nAbs) of HIV entry, the entry/fusion processes of PsVLPs are blocked, thereby preventing HiBiT release, and no luminescent signal is produced. 3. In the absence of inhibitors or nAbs, HIV HiBiT-PsVLPs bind to target cells via CD4, CCR5 or CXCR4 and undergo membrane fusion. HiBiT is released into target cells and binds to LgBiT to generate a luminescent signal.
Assay Workflow: Standardized Reagents to Improve Your Results
- Thaw-and-use cell format provides significant time and labor savings over traditional methods while reducing variability.
- Complete Kit includes all of the required reagents in standardized formats.
- Easily amenable to high-throughput workflows.
Assay Performance Data
HIV HiBiT-PsVLP Bioassay is suitable for blocking entry of nAbs and small-molecule inhibitors. HIV (LgBiT) Target cells were incubated with either anti-CD4 antibodies (Panel A) or the anti-viral temsavir (Panel B), binding to gp120 to block the entry of HIV PsVLPs. After incubation, the Bio-Glo-NB™ VLP Reagent was added and luminescence determined in a GloMax® Discover Plate Reader.
Interested in HiBiT PsVLP Bioassays for other viruses?
Contact us to learn about our bioassay development services.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
Bio-Glo-NB™ VLP Luciferase Substrate |
CS329542 | 2 × 125μl |
Bio-Glo-NB™ VLP Luciferase Assay Buffer |
CS329543 | 2 × 2.5ml |
Bio-Glo-NB™ DrkBiT Peptide (1000X) |
CS329549 | 1 × 30μl |
HIV Env (JRFL) HiBiT-PsVLPs |
CS3368A23 | 1 × 1 each |
HIV HEK293T (LgBiT) Target Cells |
CS3368A26 | 1 × 0.5ml |
Fetal Bovine Serum |
J121A | 1 × 4ml |
DMEM with 4.5g/L Glucose, L-Glutamine, Sodium Pyruvate |
J236A | 1 × 60ml |
SDS
Search for SDSCertificate of Analysis
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
This material may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes, but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this material by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this material for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or nucleotide sequence encoding the LgBiT and/or HiBiT peptides which sequences have been genetically engineered in this material. In addition, recipient must use Bio-Glo-NB™ VLP Luciferase Assay System purchased from Promega for all luminescence assays using this material or contact Promega to obtain a license for use of this material with reagents other than Promega’s.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Bio-Glo-NB™ VLP Luciferase Substrate |
CS329545 | 1 × 1.25ml |
Bio-Glo-NB™ VLP Luciferase Assay Buffer |
CS329546 | 1 × 25ml |
Bio-Glo-NB™ DrkBiT Peptide (1000X) |
CS329549 | 5 × 30μl |
HIV Env (JRFL) HiBiT-PsVLPs |
CS3368A23 | 5 × 1 each |
HIV HEK293T (LgBiT) Target Cells |
CS3368A26 | 5 × 0.5ml |
Fetal Bovine Serum |
J121A | 5 × 4ml |
DMEM with 4.5g/L Glucose, L-Glutamine, Sodium Pyruvate |
J236A | 5 × 60ml |
SDS
Search for SDSCertificate of Analysis
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
This material may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes, but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this material by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this material for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or nucleotide sequence encoding the LgBiT and/or HiBiT peptides which sequences have been genetically engineered in this material. In addition, recipient must use Bio-Glo-NB™ VLP Luciferase Assay System purchased from Promega for all luminescence assays using this material or contact Promega to obtain a license for use of this material with reagents other than Promega’s.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Part # | Size |
|---|---|---|
HIV Env (JRFL) HiBiT-PsVLPs |
CS3368A23 | 1 × 1 each |
SDS
Search for SDSCertificate of Analysis
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
This material may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes, but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this material by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this material for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or nucleotide sequence encoding the LgBiT and/or HiBiT peptides which sequences have been genetically engineered in this material. In addition, recipient must use Bio-Glo-NB™ VLP Luciferase Assay System purchased from Promega for all luminescence assays using this material or contact Promega to obtain a license for use of this material with reagents other than Promega’s.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Part # | Size |
|---|---|---|
HIV Env (JRFL) HiBiT-PsVLPs |
CS3368A23 | 5 × 1 each |
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
This material may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes, but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this material by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this material for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or nucleotide sequence encoding the LgBiT and/or HiBiT peptides which sequences have been genetically engineered in this material. In addition, recipient must use Bio-Glo-NB™ VLP Luciferase Assay System purchased from Promega for all luminescence assays using this material or contact Promega to obtain a license for use of this material with reagents other than Promega’s.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Part # | Size |
|---|---|---|
HIV HEK293T (LgBiT) Target Cells |
CS3368A26 | 1 × 0.5ml |
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
This material may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes, but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this material by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this material for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or nucleotide sequence encoding the LgBiT and/or HiBiT peptides which sequences have been genetically engineered in this material. In addition, recipient must use Bio-Glo-NB™ VLP Luciferase Assay System purchased from Promega for all luminescence assays using this material or contact Promega to obtain a license for use of this material with reagents other than Promega’s.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Part # | Size |
|---|---|---|
HIV HEK293T (LgBiT) Target Cells |
CS3368A26 | 5 × 0.5ml |
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
This material may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes, but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this material by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this material for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or nucleotide sequence encoding the LgBiT and/or HiBiT peptides which sequences have been genetically engineered in this material. In addition, recipient must use Bio-Glo-NB™ VLP Luciferase Assay System purchased from Promega for all luminescence assays using this material or contact Promega to obtain a license for use of this material with reagents other than Promega’s.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Part # | Size |
|---|---|---|
HIV HEK293T (LgBiT) Target Cells (CPM) |
CS3368A31 | 2 × 1ml |
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
This material may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes, but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this material by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this material for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or nucleotide sequence encoding the LgBiT and/or HiBiT peptides which sequences have been genetically engineered in this material. In addition, recipient must use Bio-Glo-NB™ VLP Luciferase Assay System purchased from Promega for all luminescence assays using this material or contact Promega to obtain a license for use of this material with reagents other than Promega’s.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
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