QuantiFluor® dsDNA System

Simple, Accurate DNA Quantitation

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The system is suitable for use in many molecular biology applications, including next-gen sequencing and other techniques where accurate DNA quantitation is important. This dye shows minimal binding to single stranded DNA (ssDNA), RNA, protein and interfering compounds, allowing specific quantitation of dsDNA.

Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems

The QuantiFluor® dsDNA Dye offers greater sensitivity over absorbance at 260nm (NanoDrop®) low-concentration samples, and better or equal sensitivity performance when compared to PicoGreen®. 10pg/µl vs. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume).

	Sample*	Assay
QuantiFluor® dsDNA System	0.01–200ng/µl	0.01–200ng
Quant-iT™ PicoGreen	0.02–200ng/µl	0.02–200ng
Qubit® dsDNA HS	0.2–100ng/µl	0.2–100ng
NanoDrop® 2000 Spectrophotometer	2–12,000ng/µl	2–12,000ng

*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input dsDNA per assay.

Better Sensitivity than Quant-iT™ PicoGreen®, Equivalent Linearity

The QuantiFluor® dsDNA System detects as little as 50pg/ml dsDNA in a 96-well microplate assay (1µl of a 10ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.

Proven for DNA Quantitation in Next-Gen Sequencing

Many laboratories have published papers using QuantiFluor® dyes in sequencing applications.

View Sample Citations

Easy Setup on Microplate or Single-Tube Fluorometers

System includes all the necessary reagents to quickly set up and quantitate dsDNA, and is easy to set up on microplate or single tube fluorometers, including the following Promega fluorometers.

Quantus Nucleic Acid Quantification Fluorometer

Quantus™ Fluorometer

(Use Blue Channel Ex 460nm, Em 515–575nm)

Discover Nanodrop Spectrophotometer DNA Extraction

GloMax® Discover

Explorer DNA Purification & Quantification Kit

GloMax® Explorer

How do fluorescent dyes compare to other quantitation methods?

In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.

UV-Absorbance vs Fluorescence

Fluorescence vs qPCR

Protocols

Complete Protocol

QuantiFluor® dsDNA System Technical ManualPDF (1765 KB) – English

Quick Protocols

QuantiFluor® dsDNA System Quick Protocol FB197PDF (732 KB) – English

What's in the box?

Item	Part #	Size	Available Separately
QuantiFluor® dsDNA Dye	E258A	1 × 1ml
Lambda DNA Standard	E259A	1 × 100μg
20X TE Buffer (pH 7.5)	E260A	1 × 25ml	View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.