Notes: PC12 cells were plated and deprived of oxygen and glucose for 5-7 hours. Parallel samples were assessed for viability with the CellTiter-Glo® Cell Viability Assay and cytotoxicity with CellTox™ Green Cytotoxicity Assay (end-point method).  Deprived cells were also analyzed by RT-PCR following RNA extraction with the SV Total RNA Isolation System and converted to cDNA with the ImProm-II™ Reverse Transcriptase in the presence of RNasin® Ribonuclease Inhibitor and PCR performed with the PCR Master Mix for a limited number of cycles.  (4647)

Notes: The effect of tryptophan on skin hTERT immortalized normal keratinocytes was assessed at 24 and 48 hours with the RealTime-Glo™ Cell Viability Assay.  After the read at 48 hours, CellTox™ Green Cytotoxicity Assay was added to each well to measure dead cells per well using the end-point protocol. Tryptophan, which was determined toxic to Pseudomonas biofilms, was not cytotoxic to the human keratinocytes. (4644)

Notes: A phenotypic screen of a 25,000 compound library for inhibitors of U2OS osteosarcoma cell line growth identified two compounds that showed activity of osteosarcomas and not HepG2 or primary human osteoblasts.  Cell Viability in the primary and secondary screens was monitored with the CellTiter-Blue® Cell Viability Assay.  Subsequent studies identified the mechanism of cell death as apoptosis as judged by the Caspase-Glo® 3/7 Assay and cytotoxicity measured with the CellTox™ Green Cytotoxicity Assay.
(4649)

Notes: The CellTox™ Green Cytotoxicity Assay was used to assess the viability of Leshmania amazonensis promastigotes treated with varying concentrations of auranofin. CellTox™ Green was added prior to plating cells in 384-well plates and compound treatment with both positive and negative controls. Fluorescence was read after 48 hours. Induction of L. amazonesis apoptosis by auranofin was also assessed with the Apo-ONE® Homogeneous Caspase 3/7 Assay. (4706)

Notes: The CellTox™ Green Cytotoxicity Assay was used to examine whether a bactericidal compound, Pentobra, was toxic to mammalian cells. Concentrations of up to 100µM Pentobra did not lead to significant toxicity of NIH3T3 cells. The data obtained with CellTox™ Green agreed with visual staining of cells with propidium iodide. (4705)